| ELISA对毒理实验动物血清中神经生长因子抗体动态的研究 |
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| 引用本文： | 沈心亮,苏彩霞,史中信,扈晶.ELISA对毒理实验动物血清中神经生长因子抗体动态的研究[J].兰州大学学报(自然科学版),2000,36(3):158-160. |
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| 作者姓名： | 沈心亮 苏彩霞 史中信 扈晶 |
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| 作者单位： | 卫生部,兰州生物制品研究所,甘肃,兰州,730046 |
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| 摘 要： | 用ＥＬＩＳＡ法对神经生长因子（ＮＧＦ）毒理实验动物犬和大鼠血清作了抗－ＮＧＦ抗体检测。结果表明在大鼠血清中没有发现抗－ＮＧＦ抗体，但犬血清中有相应的抗体产生，并随注射剂量增加和注射时间延长而逐渐升高，另外，对ＥＬＩＳＡ间接法和竞争抑制法进行了比较，结果表明，竞争抑制法明显优于间接法，而且是一种简便、特异的好方法。
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| 关 键 词： | 神经生长因子 抗体 ELISA 血清 毒理实验动物 |
| 修稿时间： | ： |
The Anti-NGF Antibody in Serum of Toxicological Experimental Animals Determined by ELISA |
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| Shen Xinliang,Su Caixia,Shi Zhongxin,Hu Jin.The Anti-NGF Antibody in Serum of Toxicological Experimental Animals Determined by ELISA[J].Journal of Lanzhou University(Natural Science),2000,36(3):158-160. |
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| Authors: | Shen Xinliang Su Caixia Shi Zhongxin Hu Jin |
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| Abstract: | The enzyme linked imm un osorbent assay was used to determine the anti NGF antibody in serum of dogs and rats .It was found that there was no anti NGF antibody in serum of rats, but t he antibody existed in serum of dogs .The antibody concentration increased with larger injection doze and longer time length. In addition, indirect ELISA was co mpared with the competitive inhibition of ELISA. The result indicated that the l atter is superior to the former and the competitive inhibition of ELISA is a sim ple, good method. |
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| Keywords: | nerve growth factor(NGF ) anti NGF antibody enzyme linked immunosorbent assay(ELISA) |
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