| 产R-肾上腺素脱氢酶菌种筛选与产酶条件研究 |
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| 引用本文： | 杨凯,郭黛苹,李忠琴,陈倍,陈必钦,许小平.产R-肾上腺素脱氢酶菌种筛选与产酶条件研究[J].福建师范大学学报(自然科学版),2007,23(3):67-70. |
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| 作者姓名： | 杨凯 郭黛苹 李忠琴 陈倍 陈必钦 许小平 |
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| 作者单位： | 福州大学化学化工学院,福建,福州,350002 |
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| 摘 要： | 以肾上腺酮为底物,在1 300余份土壤中筛选到一株不对称还原肾上腺酮生成R-肾上腺素的菌株S289,产物得率为23.3%,对映体过量值e.e.高达99%,并对其产酶条件进行了研究,使其酶活和比酶活分别提高了168%和230%.
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| 关 键 词： | 筛选 R-肾上腺素脱氢酶 生物转化 |
| 文章编号： | 1000-5277（2007）03-0067-04 |
| 修稿时间： | 2006-10-30 |
Screening of Strain S289 with the Ability of Biosynthesis of R-adrenalin and the Study of R-adrenalin Dehydrogenase |
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| YANG Kai,GUO Dai-ping,LI Zhong-qin,CHEN Bei,CHEN Bi-qin,XU Xiao-ping.Screening of Strain S289 with the Ability of Biosynthesis of R-adrenalin and the Study of R-adrenalin Dehydrogenase[J].Journal of Fujian Teachers University(Natural Science),2007,23(3):67-70. |
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| Authors: | YANG Kai GUO Dai-ping LI Zhong-qin CHEN Bei CHEN Bi-qin XU Xiao-ping |
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| Institution: | Department of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China |
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| Abstract: | A new strain S289 was isolated from soil using corticosterone as the substrate,which can biosynthesize R-adrenalin by asymmetric bioreduction of corticosterone,the yield and the enantiomeric excess of R-adrenalin were 23.3% and 99%.After study of the conditions of dehydrogenase production,the total activity and specific activity were enhanced 168% and 230% respectively. |
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| Keywords: | screening R-adrenalin dehydrogenase biotransformation |
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