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还原型牛胰岛素在疏水界面上的折叠
引用本文:白泉,孔宇,张养军,耿信笃.还原型牛胰岛素在疏水界面上的折叠[J].西北大学学报,2000,30(2):126-130.
作者姓名:白泉  孔宇  张养军  耿信笃
作者单位:西北大学,现代分离科学研究所,现代分离科学陕西省重点实验室,陕西,西安,710069  
基金项目:国家自然科学基金资助项目!(39880 0 0 3)
摘    要:用疏水色谱(HPHIC)对还原变性的牛胰岛素在疏水界面上的折叠规律进行了探讨,对于未来原的胍变和脲变insulin来说,胍变insulin用HPHIC法可以完全复性,而脲变insulin只能部分复性。对于还原变性的insulinHPHIC对胍变、脲变insulin只能部分复性。在非氧化型流动相中,它们的复性情况较差,而采用氧化型流动相,有助于二硫键的正确对接,可大大提高胍变和脲变insulin的复

关 键 词:蛋白折叠  疏水界面  二硫键配对  牛胰岛素  还原型
修稿时间:1999-10-21

Studies on the refolding of reduced-denatured insulin on a hydrophobic surface
BAI Quan,KONG Yu,ZHANG Yang-jun,GENG Xin-du.Studies on the refolding of reduced-denatured insulin on a hydrophobic surface[J].Journal of Northwest University(Natural Science Edition),2000,30(2):126-130.
Authors:BAI Quan  KONG Yu  ZHANG Yang-jun  GENG Xin-du
Abstract:The refolding of redcuced- denatured insulin from bovine pancreas on the hydrophobic surface was studied with high performance hydrophobic interaction chromatography ( HPHIC) .It was shown that insulin denatured with 7.0 mol/L guanidine hydrochloride( Gua HCl) can be renatured completely with HPHIC,but urea- unfolded insulin only can berefolded partly.The reduced- denatured insulin also can be refolded partly with HPHIC.In the presence of oxidized glutathione( GSSG) in the mobile phase employed,the disulfide exchange of reduced- denatured insulin can be accelerated and the threedisulfide bridgesof insulin were formed correctly. The resultshowed thatthe refolding yeild ofreduced- denatured insulin with Gua HClor urea should beincreased higher.In addition,the intermediate of urea- unfolded insulin was found and separated with HPHIC.
Keywords:hydrophobic interaction chromatography  protein folding  insulin  protein denaturation  disulfide  bonding
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