| 还原型牛胰岛素在疏水界面上的折叠 |
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| 引用本文： | 白泉,孔宇,张养军,耿信笃.还原型牛胰岛素在疏水界面上的折叠[J].西北大学学报,2000,30(2):126-130. |
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| 作者姓名： | 白泉 孔宇 张养军 耿信笃 |
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| 作者单位： | 西北大学,现代分离科学研究所,现代分离科学陕西省重点实验室,陕西,西安,710069
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| 基金项目： | 国家自然科学基金资助项目!(39880 0 0 3) |
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| 摘 要： | 用疏水色谱（ＨＰＨＩＣ）对还原变性的牛胰岛素在疏水界面上的折叠规律进行了探讨，对于未来原的胍变和脲变ｉｎｓｕｌｉｎ来说，胍变ｉｎｓｕｌｉｎ用ＨＰＨＩＣ法可以完全复性，而脲变ｉｎｓｕｌｉｎ只能部分复性。对于还原变性的ｉｎｓｕｌｉｎＨＰＨＩＣ对胍变、脲变ｉｎｓｕｌｉｎ只能部分复性。在非氧化型流动相中，它们的复性情况较差，而采用氧化型流动相，有助于二硫键的正确对接，可大大提高胍变和脲变ｉｎｓｕｌｉｎ的复
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| 关 键 词： | 蛋白折叠 疏水界面 二硫键配对 牛胰岛素 还原型 |
| 修稿时间： | 1999-10-21 |
Studies on the refolding of reduced-denatured insulin on a hydrophobic surface |
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| BAI Quan,KONG Yu,ZHANG Yang-jun,GENG Xin-du.Studies on the refolding of reduced-denatured insulin on a hydrophobic surface[J].Journal of Northwest University(Natural Science Edition),2000,30(2):126-130. |
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| Authors: | BAI Quan KONG Yu ZHANG Yang-jun GENG Xin-du |
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| Abstract: | The refolding of redcuced- denatured insulin from bovine pancreas on the hydrophobic surface was studied with high performance hydrophobic interaction chromatography ( HPHIC) .It was shown that insulin denatured with 7.0 mol/L guanidine hydrochloride( Gua HCl) can be renatured completely with HPHIC,but urea- unfolded insulin only can berefolded partly.The reduced- denatured insulin also can be refolded partly with HPHIC.In the presence of oxidized glutathione( GSSG) in the mobile phase employed,the disulfide exchange of reduced- denatured insulin can be accelerated and the threedisulfide bridgesof insulin were formed correctly. The resultshowed thatthe refolding yeild ofreduced- denatured insulin with Gua HClor urea should beincreased higher.In addition,the intermediate of urea- unfolded insulin was found and separated with HPHIC. |
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| Keywords: | hydrophobic interaction chromatography protein folding insulin protein denaturation disulfide bonding |
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