水提三七达玛烷20(S)-原人参二醇型皂苷诱导A549细胞凋亡机制的研究

三七总甙对多种肿瘤细胞的生长均有抑制作用,但从三七茎叶、花梗、果梗提取到的达玛烷20(S)-原人参二醇型皂苷(20(S)-PPDS)对肺癌A549细胞的作用及机理尚不清楚.本研究采用噻唑蓝(MTT), Hoechst33258 染色,免疫组化以及Western blot法研究20(S)-PPDS对肺癌A549细胞的作用.结果显示20(S)-PPDS对A549细胞的具有抑制作用,125,250,500 μg·mL-1的20(S)-PPDS与A549细胞作用72 h后,细胞抑制率依次为28.67%,41.97%,73.02%;20(S)-PPDS对A549细胞作用24,72 h的IC50分别为398.94,315.96 μg·mL-1;20(S)-PPDS通过活化Caspase-3, 上调Bax蛋白表达,下调Pro-Caspase-3、Bcl-2蛋白表达诱导A549细胞凋亡.这些研究结果表明20(S)-PPDS对A549细胞具有抑制细胞增殖和诱导细胞凋亡的作用.

Effects of 20(s)-protopanaxadiol on apoptosis of human pulmonary adenocarcinoma A549 cells and its mechanism

Panax notogingseng saponins inhibits the growth of several cancer cell lines,but the inhibition of 20(S)-protopanaxadiol saponins (20(S)-PPDS), an aqueous extraction group from stem,leaves,pedicel,and fruit stem of Pannax notoginseng,on the proliferation of lung adenocarcinoma cell line A549 and its possible molecular mechanism is not clear.After treating A549 cells with 20(S)-PPDS,we detected the effects of 20(S)-PPDS on A549 cells by MTT assay,immunohistochemistry,Hoechst33258 staining,and Western blot.The results showed that 20(S)-PPDS inhibited the growth of A549 cells.When treated with 125,250 and 500 μg·mL-1 of 20(S)-PPDS for 72 h,the inhibitory rates of A549 cells were 28.67%,41.97%,73.02% respectively.The 50% inhibition concentration of 20(S)-PPDS were 398.94 and 315.90 μg·mL-1 at 24 and 72 h.20(S)-PPDS induced cell apoptosis through activating Caspase-3,up-regulating Bax,down-regulating Pro-Caspase-3 and Bcl-2 protein expression.Experimental results of this study suggested that 20(S)-PPDS exerts anticancer activity through the growth inhibition and the apoptosis on A549 cells.