高效液相色谱法测定6个不同产地的牡丹叶中丹皮酚和芍药苷含量

采用高效液相色谱法测定6个不同产地来源的牡丹叶中丹皮酚和芍药苷的含量。丹皮酚与芍药苷的测定均以Waters Xbridge shield RP18色谱柱(5μm,4.6 mm×150.0 mm)为固定相,流速1.0 m L/min,柱温30℃,进样量为10μL;丹皮酚检测以甲醇-水(体积比45:55)为流动相,检测波长275 nm;芍药苷检测以乙腈-0.1%磷酸溶液(体积比15:85)为流动相,检测波长230 nm。结果显示,丹皮酚和芍药苷分别在浓度12.5～400.0μg/m L和50.0～800.0μg/m L的范围内与峰面积具有良好的线性关系,精密度、稳定性、重复性和加样回收率都较好,6个不同产地的牡丹叶中丹皮酚和芍药苷的含量有所差别。该研究为牡丹叶质量综合评价及药效的科学解释提供了准确、快速的定量分析方法,同时为探讨不同产地来源牡丹叶的化学成分及其开发利用提供了数据支持。

Determination of paeonol and paeoniflorin contents in the leaves of Paeonia suffruticosa from six growing areas using HPLC

The contents of paeonol and paeoniflorin in the leaves of Paeonia suffruticosa from six growing areas were determined using high-performance liquid chromatography (HPLC). Both chemicals were analyzed using Waters Xbridge shield RP18 chromatography columns (5 μm, 4.6 mm×150.0 mm) at 30℃ with a flow rate of 1.0 mL/min. The sample loading amount was 10 μL. For paeonol analysis, the mobile phase was methanol-water (45:55，V/V) solution with the detection wavelength set at 275 nm. For paeoniflorin analysis, acetonitrile-0.1% phosphoric acid (12:88，V/V) solution was used as the mobile phase with a detection wavelength of 230 nm. Paeonol and paeoniflorin showed good linear relationship with peak area within the concentration ranges of approximately 12.5~400.0 μg/mL and 50.0~800.0 μg/mL, respectively. The results demonstrated satisfying precision, stability, repeatability and sample recovery. The leaf contents of paeonol and paeoniflorin in Paeonia suffruticosa vary among the six growing areas. Chemical composition analysis of Paeonia suffruticosa leaves, in cases of paeonol and paeoniflorin, provides accurate and rapid quantitative analytical methods for qualitative evaluation and scientific explanation of the pharmaceutical effects. Moreover, it also provides data support for investigating the diverse chemical composition of Paeonia suffruticosa leaves from different areas, thus facilitating their exploitation and utilization.