老年雄性大鼠部分衰老指标测定的研究

目的定量研究老年雄性大鼠部分衰老指标的增龄性变化。方法测定不同月龄SD雄性大鼠血清T-SOD活性及MDA含量、血浆E2及T的含量、脑组织M受体结合容量以及左侧股骨近段、中段、远段的骨密度和右侧股骨的弯曲断裂载荷。结果30月龄雄性大鼠血清T-SOD活性和血浆E2、T含量明显低于6月龄雄性大鼠(P<0.01);30月龄雄性大鼠血清MDA含量明显高于6月龄雄性大鼠(P<0.01);30月龄雄性大鼠脑组织M受体结合容量和股骨各段骨密度及弯曲断裂载荷明显低于24月龄雄性大鼠(P<0.05)。结论30月龄雄性大鼠已显著衰老,雄性大鼠脑衰老与骨衰老有关。

Study on Part of Aging Indexes in Aged Male Rats

Objective To do quantitatively studying on some important aging indexes in aged male rats.Methods T-SOD activity,MDA level in serum,E-2 and T levels in plasma,binding capacity of M receptor,bone density of proximal,middle and distal segments of left femur,and break bending load of right femur were determined in the rats aged 6,24,and 30 months.Results T-SOD activity in serum,and E-2 and T levels in plasma of the rats aged 30 months were significantly lower than those of the rats aged 6 months(P<0.01),and MDA level in serum of the rats aged 30 months was significantly higher than that of the rats aged 6 months.The binding capacity of M receptor,and the femur bone density in respective segments and the femur break bending load of the rats aged 30 months were significantly lower than those of the rats aged 24 months(P<0.05).Conclusion The male rats aged 30 months was visibly aged and weakened,and was used as the natural aging model.The senility of the brain was closely linked to senility of bone in male rats.