露空对红树蚬生物标志物的影响

【目的】研究样品露空运输过程对红树蚬(Polymesoda erosa)生物标志物的影响。【方法】野外采集的红树蚬经8℃、26℃和35℃露空处理8h,随后在26℃海水中暂养恢复3h、6h、12h和24h,测定红树蚬血液超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、乙酰胆碱酯酶(AChE)、谷胱甘肽转硫酶(GST)活性,以及铁还原力(FRAP)和丙二醛(MDA)含量。【结果】8℃和35℃露空处理,SOD、CAT、AChE、GST、FRAP和MDA均显著升高。26℃露空处理,SOD、AChE和MDA显著升高,GST显著降低,CAT和FRAP有所降低但不显著。经过暂养,红树蚬各项指标均能恢复到初始水平,但24h内波动较大。【结论】露空对红树蚬的生物标志物有显著影响。26℃露空8h后,经3~6h暂养,红树蚬生物标志物与初始水平最接近。

The Effects of Air Exposure on Biomarkers of Mangrove Mud Clam (Polymesoda erosa)

[Objective]In this study,the effects of air exposure on responses of the biomarkers of mangrove mud clam （Polymesoda erosa ）in transit was explored.[Methods]The clams,after being exposed to air for 8h at 8℃,26℃ and 35℃,were placed back in seawater of 26℃ for 3h, 6h,12h and 24h,respectively,and then six biomarkers of the clams haemolymph were meas-ured,including superoxide dismutase activity （SOD）,catalase activity （CAT）,acetylcholinest-erase activity （AChE）,glutathione S-transferase activity （GST）,ferric reducing antioxidant power （FRAP）and malodiadehyde content （MDA）.[Results]Significant increases in SOD, CAT,AChE,GST,FRAP and MDA were observed in the clams exposed to air at 8℃ or 35℃. Significant increases in SOD,AChE and MDA,great decrease in GST,and slight decreases in CAT and FRAP were found in the clams exposed to air at 26℃.All six biomarkers recovered to their initial levels after the clams were put back in seawater for 24h,but they were found fluctuated within 24h.[Conclusion]The effects of air exposure on biomarkers of the calm were found to be significant.In all treatments,the lev-els of biomarkers of the clams exposed to air for 8h at 26℃ and then placed back in seawater for 3h to 6h were closest to their initial levels.