Transfer of bacterial blight resistance from <Emphasis Type="Italic">Oryza meyeriana</Emphasis> to <Emphasis Type="Italic">O. sativa</Emphasis> L. by asymmetric somatic hybridization
(1) National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China;(2) China National Rice Research Institute, 310006 Hangzhou, China
Abstract:
Asymmetric somatic hybrid plants were produced between cultivated rice (Oryza sativa L.) and wild species O. meyeriana (Zoll. etMor. exSteud.)] with high resistance to rice bacterial blight. X-ray-irradiated protoplasts of the wild species
were used as donor and chemically fused with iodoacetamide-inactivated protoplasts of rice cv. 02428 to produce hybrids. Seventy-two
plants were regenerated from 623 calli based on metabolic complementation. The morphological characters of the plants closely
resembled that of the rice. Simple sequence repeats were employed to identify their hybridity. Cytological analysis of root-tips
revealed that their chromosome number varied in the range of 27–38. The somatic hybrids were inoculated with strains of Xanthamonas oryzae pv. oryzae at adult growth stage and demonstrated the resistance to bacterial blight introgression from the O. meyeriana.