| 光合细菌对对氯苯胺的耐受性研究 |
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| 引用本文： | 李亮,胡筱敏,董怡华,郑玲子. 光合细菌对对氯苯胺的耐受性研究[J]. 东北大学学报(自然科学版), 2010, 31(2): 273-276. DOI: - |
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| 作者姓名： | 李亮 胡筱敏 董怡华 郑玲子 |
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| 作者单位： | 东北大学资源与土木工程学院,辽宁,沈阳,110004 |
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| 基金项目： | 国家水体污染控制与治理科技重大专项(2008ZX07208-009) |
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| 摘 要： | 从本溪钢铁焦化厂排污口附近水体浅层底泥中筛选出一株高效降解对氯苯胺(PCA)的紫色非硫光合细菌CS2,实验考察了不同质量浓度PCA对CS2(出发菌株和驯化菌株)生长及其脱氢酶活性的影响.结果表明:随着PCA质量浓度的提高,CS2出发菌的生长和脱氢酶活性会受到明显抑制.CS2驯化后,CS2菌对PCA的耐受性显著提高.采用化学品对水生生物急性毒性试验的标准方法研究了PCA对CS2菌的毒性效应.结果表明,PCA对出发菌株生长的安全质量浓度为60 mg.L-1,抑制CS2菌生长的半抑制浓度96h-IC50为162.5 mg.L-1,驯化后,菌株的安全质量浓度为100 mg.L-1,96h-IC50为423.9 mg.L-1.
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| 关 键 词： | 光合细菌 对氯苯胺(PCA) 耐受性 脱氢酶活性 半抑制浓度 |
On Tolerability of Photosynthetic Bacteria for p-Chloroaniline |
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| LI Liang,HU Xiao-min,DONG Yi-hua,ZHENG Ling-zi. On Tolerability of Photosynthetic Bacteria for p-Chloroaniline[J]. Journal of Northeastern University(Natural Science), 2010, 31(2): 273-276. DOI: - |
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| Authors: | LI Liang HU Xiao-min DONG Yi-hua ZHENG Ling-zi |
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| Affiliation: | School of Resources & Civil Engineering;Northeastern University;Shenyang 110004;China. |
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| Abstract: | A strain of purple non-sulfur bacteria(PNSB) named CS2 was screened from the sediment near the sewage outfall in Benxi Steel Coking Plant.As a photosynthetic strain,CS2 can degrade the p-chloroaniline(PCA) with high efficiency.The effects of concentration of PCA on cell growth and dehydrogenase activity of original/domesticated CS2 strains were investigated.Experimental results showed that with the increasing PCA concentration the growth and dehydrogenase activity of original bacteria are obviously inhabite... |
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| Keywords: | photosynthetic bacteria p-chloroaniline tolerability dehydrogenase activity IC50 |
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