扩展莫尼茨绦虫反应元件结合蛋白基因的克隆和cDNA序列分析

克隆扩展莫尼茨绦虫（Moniezia expansa）新基因，通过生物信息学方法分析该基因的生物学信息，初步预测该基因的功能，为进一步研究扩展莫尼茨绦虫并以其作为模式生物，为人类的某些基因未知功能的研究提供基础。构建扩展莫尼茨绦虫成虫cDNA文库，随机挑取重组阳性克降进行测序，对部分序列进行引物步移法测序，获取其全长cDNA序列；在NCBI上对该cDNA序列进行开放阅读框（ORF）的寻找、编码氨基酸的推导、核苷酸和氨基酸同源性比较以及蛋白质二级结构的初步预测。结果：获得了1个扩展莫尼茨绦虫基因－应结合蛋白，全长1861bp，编码592个氨基酸，属于DUF906家族，与曼氏血吸虫反应结合蛋白基因具有72．1％的同源性。编码蛋白的理论分子量为69．7653kDa，等电点为9．83.结论：获得了扩展莫尼茨绦虫反应结合蛋白的全长cDNA序列，对该基因功能的初步预测，它是一种基因转录因子，在扩展莫尼茨绦虫虫体中的具体作用机制目前还不清楚，推断该基因的功能在脊椎动物，乃至人类的基因功能研究中具有一定借鉴意义.

An Isolation and Sequence Analysis of the cDNA Encoding Moniezia expansa CREB-Binding Protein

To isolate and identify novel genes from an adult Moniezia expansa （M. expansa） eDNA library, and provide a foundation for further research. A eDNA library was constructed from M. expansa adult stage. Clones were selected randomly from the eDNA library and were sequenced by using the method of expression sequence tags （ ESTs）. Novel genes were acquired by primer-walking. By using bioinformatics, the eDNA sequence encoding M. expansa CREB-binding protein was analyzed,including searching the ORF,translating the nucleotide to protein sequence similarity searches and secondary structure predication. Results： CREB-binding protein genes, 1861bp and coding for 592 amino acids, was acquired, submitted to GenBank angot an accession number that was GH291476. The CREB-binding protein gene of M. expansa and Schistosoma mansoni were homologous with an identity of 72.1%. The academic pI was 9.83 and molecular weight was 69. 7653kDa. Conclusion：The full-length cDNA sequence encoding M. expansa CREB-binding protein was obtained, which gave a basis for further functional study of this gene.