皮燕麦胚性愈伤组织的形成及植株再生

本实验以皮燕麦２０７９ 成熟种子胚为外植体，其愈伤组织的诱导率最高，叶鞘愈伤组织诱导率为５ ％ ，叶片几乎不发生脱分化。在基本培养基比较中以改良Ｎ６ 培养基诱导效果为佳，愈伤组织出愈率在１－３ｍｇ／Ｌ，２ ，４—Ｄ浓度范围逐渐提高，继续增大时则出愈率降低。胚性愈伤组织形成率受脱分化培养基中２，４ —Ｄ浓度的影响。扫描电镜观察结果显示非胚性与胚性愈伤组织表面结构差异明显；组织切片表明形成的再生植株有胚胎发生与器官发生两种途径，形态上有区别。

Embryogenic Callus Formation And Plantlet Regeneration Of Oats

The efficiency of callus induction was much higher,if mature embryos of oats were used as explants.No dedifferentiation was observed by using leaf blade as explants.The best medium was modified N 6.The efficiency of callus raise when 2,4-D concentration increased from 1 to 3 mg/L.Overhigh concentration of 2,4_D was worse.The concentration of 2,4-D in dedifferentiation medium had relation to embryogenie callus formation.It was different bettew the surface structure of the embryogenic and non-embryogenic calluses by means of observation by scanning electron microscope.The callus sections showed That the Plantlet regeneration was differentiated ways.