| 微波辅助酶法水解草鱼鱼鳞的工艺条件 |
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| 引用本文： | 曹光辉,黄诚,尹红,李永平.微波辅助酶法水解草鱼鱼鳞的工艺条件[J].吉首大学学报(自然科学版),2010,31(2):101-104. |
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| 作者姓名： | 曹光辉 黄诚 尹红 李永平 |
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| 作者单位： | （吉首大学生物资源与环境科学学院，湖南 吉首 416000) |
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| 摘 要： | 研究微波萃取技术对木瓜蛋白酶水解草鱼鱼鳞条件的影响，探讨在一定的微波功率和辐射时间下，酶用量、底物浓度、酶解温度及酶解时间对水解度的影响，单因素试验确定较好的因素水平，正交试验确定最佳提取工艺条件.结果表明，在设定微波功率和辐射时间为400 W 60 s时，酶法水解草鱼鳞最佳工艺条件是：酶用量5 g/L、底物浓度20%、酶解温度60 ℃和酶解时间1 h.
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| 关 键 词： | 微波辅助 酶法水解 鱼鳞 木瓜蛋白酶 |
Conditions of Microwave-Assisted hydrolization of Grass Carp Scale with Papain |
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| CAO Guang-hui,HUANG Cheng,YIN Hong,LI Yong-ping.Conditions of Microwave-Assisted hydrolization of Grass Carp Scale with Papain[J].Journal of Jishou University(Natural Science Edition),2010,31(2):101-104. |
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| Authors: | CAO Guang-hui HUANG Cheng YIN Hong LI Yong-ping |
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| Institution: | （College of Biology and Environmental Sciences,Jishou University,Jishou 416000，Hunan China） |
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| Abstract: | The impact of microwave extraction technique on papain hydrolyzing grass carp scale is investigated.Under certain conditions of microwave power and microwave radiation time,how enzyme dosage,substrate concentration,enzyme temperature and time influence degree of hydrolysis is discussed.The best extraction process conditions are determined by preferable level of factor which is decided by univariate test as well as orthogonal experiment.The results show that the best technological conditions of enzyme hydoly... |
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| Keywords: | Microwave-Assisted enzyme hydrolization scale papain |
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