Identification of two-dimensional electrophoresis-separated proteins in human hepatoma cell by electrospray ion trap mass spectrometry |
| |
Authors: | Rong Zeng Lirong Yu Xiaoxia Shao Qichang Xia |
| |
Institution: | (1) Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 200031 Shanghai, China |
| |
Abstract: | As one of the most important analytical methods in proteome research, mass spectrometry was utilized to identify proteins
separated by two-dimensional electrophoresis in the human hepatoma cell line BEL-7404. The protein spots were excised from
the gel, followed by in-gel digestion, and the peptide mappings were analyzed by liquid chromatography electrospray ion trap
mass spectrometer. Nine proteins were identified via database searching, according to the molecular weights and amino acid
sequences of peptides, among which two proteins have not been identified in the other liver-cell database. The sequence coverage
was 21% –72%. Furthermore, the relationship between the expressed proteins and the liver carcinoma was discussed. |
| |
Keywords: | ion trap mass spectrometry liver hepatoma cell proteome |
本文献已被 万方数据 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |