Resolution of small G1 and G2 populations of L1210 leukemia by flow microfluorometric analysis aided by velocity sedimentation |
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Authors: | M Edelstein P Lelieveld |
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Institution: | (1) Radiobiological Institute, T. N. O., Rijswijk, (The Netherlands);(2) Present address: Section of Cancer Biology, Division of Radiation Oncology, Mallinckrodt Institute of Radiology, Washington University School of Medicine, 63110 St. Louis, Mo., USA |
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Abstract: | Summary L1210 leukemic cells grown in vitro were subjected to kinetic analysis using a flow microfluorometer. A single broad peak was found for the DNA content distribution if unfractionated cells were used; prior fractionation using lg velocity sedimentation allowed the separation of small peaks with smaller (G1) and larger (G2) DNA contents from the dominant S phase peak with intermediate DNA content.This work was supported by grant number 5P01CA13053 awarded by the National Cancer Institute, DHEW USA, and by grant num ber RBI 76-1 from the Queen Wilhelmina Fund, The Netherlands. |
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