On the problem of linear incorporation of amino acids into cell protein |
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Authors: | D N Wheatley |
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Institution: | (1) Cell Pathology Laboratory, Department of Pathology, University Medical Buildings, Foresterhill, AB92ZD Aberdeen, (Scotland) |
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Abstract: | Summary Incorporation of amino acids into mammalian cell protein shows immediate linear kinetics when measured at intervals as small as 5 sec. Free amino acids equilibrate instantaneously across the cell membrane, acting as the precursor supply. Glycine, which does not initially show linear kinetics, may have a significant endogenous precursor supply, but this is not the acid-soluble pool.Acknowledgments. I thank the University of Aberdeen Medical Faculty Endowment Fund for support, and Mrs J. Robertson for technical assistance. |
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