| B→O blood conversion |
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| 引用本文： | ZGHANYangpei LANJiongcai 等.B→O blood conversion[J].中国科学通报(英文版),2003,48(3):269-273. |
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| 作者姓名： | ZGHANYangpei LANJiongcai |
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| 作者单位： | [1]InstituteofBloodTransfrusion,AcademyofMilitaryMedicalSciences,Beijing100850,China [2]SouthHospital,TheFirstMilitaryMedicalUniversity,Guangzhou510515,China |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家重点基础研究发展计划(973计划)，解放军重点项目 |
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| 摘 要： | To maintain a constant supply of “universal blood type”,or group O red blood cells,has benefit in specialized transfusion condition.In this study, α-galactosidase cDNA was cloned from Catimor coffee bean grown in Hainan island,China,by the RT-PCR method.We have constructed a vector for α-galactosidase cDNA expression and transferred α-galactosidase cDNA into Pichia pastoris GS115 cells by electroporation.The recombinant α-galactosidase(r-α-GalE)was purified by cation ion exchange chromatography.After studying the biochemical characters of r-α-GalE we have succeeded in converting human erythrocytes from group B to group O.The animal experiment showed that transfusion of enzymetically converted group O red blood cells(ECORBC)was perfectly safe.
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| 关 键 词： | 红细胞血型 B型 血型转化 O型 重组α-半乳糖苷酶 |
| 收稿时间： | 2002-10-17 |
B→O blood conversion |
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| Yangpei Zhang,Jun Yang,Xin Gao,Yanjun Jia,Shouping Ji,Feng Gong,Zepeng Liu,Huiming Ren,Subo Li,Jiongcai Lan,Qiong Cao.B→O blood conversion[J].Chinese Science Bulletin,2003,48(3):269-273. |
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| Authors: | Yangpei Zhang Jun Yang Xin Gao Yanjun Jia Shouping Ji Feng Gong Zepeng Liu Huiming Ren Subo Li Jiongcai Lan Qiong Cao |
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| Institution: | ZHANG Yangpei1, YANG Jun1, GAO Xin1, JIA Yanjun1, JI Shouping1, GONG Feng1, LIU Zepeng1, REN Huiming1, LI Subo1, LAN Jiongcai2 & CAO Qiong2 1. Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China; 2. South Hospital, The First Military Medical University, Guangzhou 510515, China |
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| Abstract: | To maintain a constant supply of universal blood type, or group O red blood cells, has benefit in specialized transfusion condition. In this study, a-galactosidase cDNA was cloned from Catimor coffee bean grown in Hainan island, China, by the RT-PCR method. We have constructed a vector for a-galactosidase cDNA expression and transferred a-galactosidase cDNA into Pichia pastoris GS115 cells by elec-troporation. The recombinant a-galactosidase (r-a-GalE) was purified by cation ion exchange chromatography. After studying the biochemical characters of r-a-GalE, we have succeeded in converting human erythrocytes from group B to group O. The animal experiment showed that transfusion of enzymetically converted group O red blood cells (ECORBC) was perfectly safe. |
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| Keywords: | B→O blood conversion recombinant a-galactosidase |
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