Chromatosomes are not produced fromTetrahymena chromatin by micrococcal nuclease digestion |
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| Authors: | M Suda |
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| Institution: | (1) Institute of Endocrinology, Gunma University, 371 Maebashi Gunma, Japan |
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| Abstract: | Summary The subnucleosomal organization ofTetrahymena chromatin, which has an unusual H1 histone, was investigated by NaCl extraction and micrococcal nuclease digestion of nuclei. It was found thatTetrahymena histone H1 is extracted with 0.35 M NaCl, whereas bovine thyroid H1 is not. Micrococcal nuclease digestion ofTetrahymena nuclei did not yield chromatosomes as a stable intermediate, whereas digestion of bovine thyroid nuclei did. |
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| Keywords: | Tetrahymena chromatin chromatosome histone H1 |
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