陡脉冲肿瘤治疗仪的研制及应用

在一定电场强度陡脉冲的作用下，细胞膜会形成大量微孔，其数量和孔径会随电场强度的增加而激增，从而引起膜组织断裂，导致细胞死亡，这种现象被称为不可逆性电击穿，为了研究陡脉冲对肿瘤细胞的不可逆性电击穿，应用现代电力电子技术和高电压新技术，研制出了一套脉冲峰值、脉冲宽度、脉冲重复频率和脉冲陡度独立可调的能量可控陡脉冲肿瘤治疗仪。分析了该治疗仪各部分电路的工作原理，在实验室调试成功的基础上，应用该仪器对人卵巢腺癌SKOV3细胞进行了细胞实验，调试时的示波器实测波形和细胞实验的电镜结果表明，该治疗仪性能稳定，能够方便地调节输出脉冲的能量，能有效地杀伤肿瘤细胞。

Development and Application of Steep Pulse Apparatus for Tumor Treatment

Under a certain electric field strength of steep pulse, plenty of micropores occur in the cell membrane. The number and diameter of micropore increase rapidly with the increase of electric field strength, the membrane breaks and the cell dies at last. This phenomenon is called as irreversible electrical breakdown (IREB). To research IREB of tumor cell, integrated with power electronics and conventional high voltage pulse generator, an energy-controllable steep pulse apparatus for tumor treatment is developed. The peak value, width, frequency and rise time of the output pulse can be adjusted independently, so the energy of the output pulse can be easily controlled. The apparatus is applied in cell experiment after a detailed analysis of its operating principle and debugging in laboratory. The experiment results show that the device works steadily and satisfies the demand of medical experiment.