柞蚕微孢子虫长极丝型孢子总蛋白的鉴定及功能分析

柞蚕（Antheraea pernyi Guerin—Meneville）是中国北方重要的吐丝经济昆虫，柞蚕微孢子虫（N0sema anthgmPne）引起的柞蚕微粒子病是柞蚕产业毁灭性的病害。本研究采用梯度Percoll—NaCl溶液分离纯化柞蚕微孢子虫，提取柞蚕微孢子虫总蛋白，胰蛋白酶消化，然后通过LC—MS／MS精确获得各肽段分子量，经数据库比对后共鉴定到610个蛋白。对鉴定到的蛋白质进行GO蛋白质功能分析，发现孢子时期参与大分子复合物及结构分子活性的蛋白的较多，而参与细胞催化和绑定功能、细胞过程及代谢过程的表达蛋白的所占的比例明显低于预测基因的的比例。研究认为这一结果主要与孢子期的微孢子虫脱离宿主细胞，生理不活跃，处于相对静止的时期，没有能量和物质来源有关；微孢子虫通过降低细胞活动和物质代谢，从而适应所处的环境。

Identification and Functional Analysis of a Long Pole Tussah of the Total Protein of Nosema antheraeae

Antheraea pernyi Guerin-Meneville is a very important economic insect in northern China, pebrine disease caused by Nosema antheraeae is a devastating disease. In this study, gradient Percoll-NaC1 solutions were used to separate and purify the N. antheraeae. The total protein of N. antheraeae was extracted and digested by trypsin, and then get the exact segment molecular weight of each peptide by LC-MS/MS, more than 610 proteins were obtained against database. GO protein function analysis was carried out; much protein is participating macromolecular complexes and structure. The proportion of catalytic activity, binding, cellular process and metabolic process were lower than the proportion of predicted genes. We considered mainly because it departs from the host cell in spore stage, cell physiology is inactive. Therefore, N. antheraeae adapted to the harsh environment by reduc- ing the cell activities and material metabolism.