Photodynamic sensitizers assay: rapid and sensitive iodometric measurement |
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| Authors: | J Mosinger B Mosinger |
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| Institution: | (1) Faculty of Science Department of Inorganic Chemistry, Charles University, 2030 Hlavova, 12840 Prague 2, (Czech Republic);(2) Institute for Clinical and Experimental Medicine, 800 Videnska, 14000 Prague 4, (Czech Republic) |
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| Abstract: | A rapid, sensitive and simple spectrophotometric method for the detection of1O2 produced by photodynamic photosensitizers in slightly acid and air-saturated aqueous solutions has been developed. The method is based on the reaction of1O2 (produced by photodynamic processes) with I– in the presence of ammonium molybdate as a catalyst. The reaction product I3
–, proportional to1O2, is followed spectrophotometrically at 355 nm. Several ways of avoiding interference with other oxidizing compounds, either present before or produced during the irradiation, are described.The method could be used to measure the efficiency of water-soluble photodynamic photosensitizers. |
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| Keywords: | Photosensitizers singlet oxygen iodometric measurement |
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