| 原癌基因c-raf在精原干细胞中的表达及作用 |
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| 引用本文： | 闫登科,徐斯凡,王晶磊,陈家详.原癌基因c-raf在精原干细胞中的表达及作用[J].中央民族大学学报(自然科学版),2007,16(3):233-237. |
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| 作者姓名： | 闫登科 徐斯凡 王晶磊 陈家详 |
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| 作者单位： | 1. 南昌大学,医学院,南昌,330006
2. 中央民族大学,生命与环境科学学院、中国少数民族传统医学研究中心,北京,100081 |
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| 基金项目： | 国家自然科学基金;江西省科技攻关计划;江西省教育厅科研项目 |
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| 摘 要： | 本研究用基因测序和BLAST检验扩增的精原干细胞DNA和c-raf的同源性;用MTT法观察c-rafASODNs(反义c-raf寡脱氧核甘酸)拮抗c-raf对体外培养精原干细胞培养的作用,探讨原癌基因c-raf对体外精原干细胞培养增殖的影响.实验结果显示扩增的精原干细胞DNA和c-raf的同源性98%;c-rafASODNs对体外培养的精原干细胞存活呈剂量依赖性抑制作用(P<0.05).提示c-raf在精原干细胞中表达;原癌基因c-raf促进精原干细胞体外培养的增殖.
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| 关 键 词： | 精原干细胞 反义寡脱氧核甘酸 |
| 文章编号： | 1005-8036（2007）03-0233-05 |
| 修稿时间： | 2007-03-06 |
The Expression and Effect of Proto-oncogenec-raf in Spermatogonial Stem Cells |
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| YAN Deng-ke,XU Si-fan,WANG Jin-lei,CHEN Jia-xiang.The Expression and Effect of Proto-oncogenec-raf in Spermatogonial Stem Cells[J].Journal of The Central University for Nationalities(Natural Sciences Edition),2007,16(3):233-237. |
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| Authors: | YAN Deng-ke XU Si-fan WANG Jin-lei CHEN Jia-xiang |
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| Institution: | 1. Medical College, Nanchang University Nanchang 330006, China ; 2. College of Life and Environmental Sciences, Central University for Nationalities, Beijing 100081, China |
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| Abstract: | DNA sequencing and BLAST were used to identify the homology between the amplified spermatogonial stem cell's DNA and c-raf.The effect of antisense oligodeoxynucleotides(ASODNs) on proliferation of spermatogonial stem cells in vitro was observed by MTT assay.The results showed that the homology between the amplified spermatogonial stem cell's DNA and the c-raf was 98% and c-raf ASODNs decreased the survival rate of spermatogonial stem cells in a does-dependent manner(P<0.05).It's suggested that c-raf was not only expressed in spermatogonial stem cell but also enhanced the survival and proliferation of spermatogonial stem cell. |
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| Keywords: | c-raf |
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