| 茶碱对美味侧耳胞外多酚氧化酶和纤维素酶活性的影响 |
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| 引用本文： | 陶树兴,薛知文,李颖辉. 茶碱对美味侧耳胞外多酚氧化酶和纤维素酶活性的影响[J]. 陕西师范大学学报(自然科学版), 1988, 0(3) |
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| 作者姓名： | 陶树兴 薛知文 李颖辉 |
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| 作者单位： | 陕西师大生物系，陕西师大生物系，陕西师大生物系 |
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| 摘 要： | 本文以美味侧耳为实验菌研究了茶碱对胞外多酚氧化酶和纤维素酶活性的影响.多酚氧化酶活性测定是用邻甲氧基苯酚为底物,用分光光度法测定.纤维素酶活性测定是以微晶纤维素和 CMC 为底物,用 DNS 法测定还原糖生成量.结果表明:当棉子壳培养基中茶碱浓度为0.5～2ppm 时,酶活提高极为显著,这一结果与茶碱促进菌丝生长和子实体形成的效应相吻合.
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| 关 键 词： | 茶碱 美味侧耳 多酚氧化酶 纤维素酶 |
EFFECT OF THEOPHYLLINE ON ACTIVITIES OF EXOCELLULAR POLYPHENOL OXIDASE AND CELLULASE IN PDEUROTUS SAPIDUS |
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| Tao Shuxing Xue Zhiwen Li Yinghui. EFFECT OF THEOPHYLLINE ON ACTIVITIES OF EXOCELLULAR POLYPHENOL OXIDASE AND CELLULASE IN PDEUROTUS SAPIDUS[J]. Journal of Shaanxi Normal University: Nat Sci Ed, 1988, 0(3) |
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| Authors: | Tao Shuxing Xue Zhiwen Li Yinghui |
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| Affiliation: | Department of Biology |
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| Abstract: | This paper has reported the effect of theophylline on activities of exocellular polyphenol oxidase and cellulase in Pleurotus sapidus.Various amounts of theophylline were added to cottonseed-shell medium.Taken O-Methoxyphenol as the substrate,the polyphenol oxidase activity was determined by the spectrophotometry.Taken microcry- stalline cellulose and CMC as the substrate,the cellulase activity was determined by measuring the product of reducing-sugar.Within the concentration used in this experi- ment,the enzymatic activities of all treatments which contained theophylline were higher than that of the control.The activities were the highest when the concentration of theophylline in the medium was from 0.5 to 2ppm.This result tallied with the effect Of theophylline on the growth of mycelia and the forming of fruiting bodies in Pleurotus sapidus. |
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| Keywords: | theophylline Pleurotus sapidus polyphenol oxidase cellulase |
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