| 女贞（Ligustrun lucidum Ait）内生真菌抑菌活性及发酵条件初探 |
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| 引用本文： | 陆伟东,田雪莲.女贞（Ligustrun lucidum Ait）内生真菌抑菌活性及发酵条件初探[J].曲靖师专学报,2013(6):36-39. |
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| 作者姓名： | 陆伟东 田雪莲 |
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| 作者单位： | 曲靖师范学院生物资源与环境科学学院,云南曲靖655011 |
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| 基金项目： | 曲靖师范学院校级课题“银杏内生真菌毒杀烟草根结线虫的研究”（2010QN014） |
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| 摘 要： | 从女贞茎、叶中分离到21株内生真菌,分别用PDA,YPD和NHK培养基摇瓶发酵培养后获得各菌株发酵液,以E.Coli,B.Subtilis作为测试菌株,寻找最优化产次生代谢产物的发酵条件,综合抑菌活性分析后得出,女贞内生真菌液体培养的最适碳源是蔗糖和淀粉,最适氮源是胰蛋白胨,摇瓶速度为120r/min,瓶装量为70ml/250ml,最适pH7.0,培养时间12天.
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| 关 键 词： | 内生真菌 发酵条件 抑菌 |
Research on Antimicrobe Activity of Ligustrun Lucidum Ait Endophytic Fungus |
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| Authors: | Lu Weidong Tian Xuelian |
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| Institution: | (Qujing Normal University, Qujing Yunnan 655011, P. R. China) |
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| Abstract: | The inhibitory activity test to three species of microbe （ Bacillus subtilis, Escherichia coli） were conducted to research the effects of different fermentation conditions on antimierobe activity of Ligustrun lucidum Ait endophytic fungus . The optimal condition possessed high antimicrobe activity reveal that sucrose and starch were suitable C source; that Tryptone was suitable for N source, a 120 r/min shake revolutions with 70 mL medium in 250 mL flask, culture temperature 28, initial pH7.0 and culture time 8 days; and that the diameter of inhibition zone was the longest. |
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| Keywords: | inhibitory activity endophytic fungi fermentation condition |
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