| 转基因小麦环境释放中基因漂移研究 |
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| 引用本文： | 汪越胜,覃建兵,李克秀,何光源.转基因小麦环境释放中基因漂移研究[J].华中科技大学学报(自然科学版),2004,32(12):91-93. |
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| 作者姓名： | 汪越胜 覃建兵 李克秀 何光源 |
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| 作者单位： | 华中科技大学,中英HUST-RRes作物遗传工程和基因组学,联合实验室,湖北,武汉,430074 |
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| 基金项目： | 国家高技术研究发展计划资助项目 (2 0 0 2AA2 2 4 0 31) |
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| 摘 要： | 基因漂移供体是转基因小麦，受体有转基因小麦的非转基因分离体、受体对照品系和地方小麦品种，以外源uidA基因表达作为基因漂移的分子标记，在供体开花期间，距供体5个不同距离上收集花粉，约80％的花粉分布在距供体中心15m以内，距离35m处只检测到约6％的花粉，在11个检测样点检测受体和供体间杂交率，供体与分离体混种同一小区时，基因漂移频率较高(1．08％)；与其受体对照播种于相邻小区(相距约0．5m)时，基因漂移频率居中(0．63％)；地方品种距供体边缘0．5～2．5m处，频率较低(0．25％)；3m以外没有检测到基因漂移，认为转基因小麦基因漂移距离应在3m以内。
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| 关 键 词： | 转基因小麦 环境释放 花粉 基因漂移 |
| 文章编号： | 1671-4512(2004)12-0091-03 |
| 修稿时间： | 2003年8月27日 |
Assessment of transgenic wheat (triticum aestivum L.) by gene flow |
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| Wang Yuesheng Qin Jianbin Li Kexiu He Guangyuan Wang Yuesheng Assoc. Prof., China-UK HUST-RRes Crop Genetic Engineering and Genomics Joint Lab.,Huazhong Univ. of Sci. & Tech.,Wuhan ,China..Assessment of transgenic wheat (triticum aestivum L.) by gene flow[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE,2004,32(12):91-93. |
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| Authors: | Wang Yuesheng Qin Jianbin Li Kexiu He Guangyuan Wang Yuesheng Assoc Prof China-UK HUST-RRes Crop Genetic Engineering and Genomics Joint Lab Huazhong Univ of Sci & Tech Wuhan China |
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| Institution: | Wang Yuesheng Qin Jianbin Li Kexiu He Guangyuan Wang Yuesheng Assoc. Prof., China-UK HUST-RRes Crop Genetic Engineering and Genomics Joint Lab.,Huazhong Univ. of Sci. & Tech.,Wuhan 430074,China. |
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| Abstract: | The field test of three transgenic wheat lines was carried out to determine the distance and frequency of pollen-mediated gene flow from transgenic wheat. The non-transgenic segregants, the target controls of transgenic lines and the local varieties of wheat were set to be the recipients. The result showed that pollen grains dispersed within 35 meters and no gene flowed beyond 3 meters. It was significant for further evaluation of the transgenic wheat. |
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| Keywords: | transgenic wheat field trail pollen grain gene flow |
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