| 烤烟品种NC297内生细菌中拮抗烟草黑胫病的生防菌筛选及种群组成分析 |
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| 引用本文： | 冯云利,奚家勤,马莉,莫明和,方敦煌,夏振远,雷丽萍,杨发祥,周峰.烤烟品种NC297内生细菌中拮抗烟草黑胫病的生防菌筛选及种群组成分析[J].云南大学学报(自然科学版),2011,33(4):488-496. |
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| 作者姓名： | 冯云利 奚家勤 马莉 莫明和 方敦煌 夏振远 雷丽萍 杨发祥 周峰 |
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| 作者单位： | 云南大学生物资源保护与利用重点实验室;中国烟草总公司郑州烟草研究院;云南省烟草农业科学研究院;云南云叶化肥股份有限公司; |
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| 基金项目： | 郑州烟草研究院科技资助项目(122009CZ0420); 国家自然科学基金资助项目(30860017) |
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| 摘 要： | 从来自烤烟品种NC297的970株内生细菌中,以烟草黑胫病菌(Phytophthora parasitica var. nicotianae) 为靶标,共筛选出165株拮抗菌,这些内生细菌对黑胫病菌的抑菌率在9.55%~55.96%之间.内生细菌的生物量在苗期、团棵期、开花期和成熟期中无显著差异,但拮抗菌数量呈上升趋势.对165株拮抗菌的16S rRNA基因序列进行RFLP分析共产生12种带型.根据RFLP带型选取41株进行16S rRNA基因序列测定和系统发育分析.结果表明这165株生防内生细菌归于3大类群:Proteobacteria,Actinobacteria和Firmicutes.Firmicutes类群中的芽孢杆菌属(Bacillus)是优势属,共6种RFLP带型,159个菌株,包括Bacillus amyloliquefaciens subsp.plantarum,Bacillus methylotrophicus,Bacillus tequilensis,Bacillus aryabhattai,Bacillus cereus和Bacillus mycoide.Bacillus amyloliquefaciens subsp.plantarum和Bacillus.methylotrophicus是Bacillus中分离频率最高的2种内生拮抗菌,共149株,占该属菌株数的90.30%.其余6个菌株分别属于6个种:Brevibacillus reuszeri,Streptomyces finlayi,Streptomyces atroolivaceus,Erwinia rhapontici,Enhydrobacter aerosaccus和Stenotrophomonas rhizophila.
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| 关 键 词： | NC297 内生细菌 生物防治 烟草黑胫病 系统发育分析 |
| 收稿时间： | 2011-3-28 |
Isolation and phylogenetic analysis of the antagonistic endophytic bacteria against Phytophthora parasitica var.nicotianae from tobacco variety NC297 |
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| FENG Yun-li,XI Jia-qin,MA Li,MO Ming-he,FANG Dun-huang,XIA Zhen-yuan,LEI Li-ping,YANG Fa-xiang,ZHOU Feng.Isolation and phylogenetic analysis of the antagonistic endophytic bacteria against Phytophthora parasitica var.nicotianae from tobacco variety NC297[J].Journal of Yunnan University(Natural Sciences),2011,33(4):488-496. |
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| Authors: | FENG Yun-li XI Jia-qin MA Li MO Ming-he FANG Dun-huang XIA Zhen-yuan LEI Li-ping YANG Fa-xiang ZHOU Feng |
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| Institution: | FENG Yun-li1,XI Jia-qin2,MA Li1,MO Ming-he1,FANG Dun-huang3,XIA Zhen-yuan3,LEI Li-ping3,YANG Fa-xiang4,ZHOU Feng5(1.Key Laboratory for Conservation and Utilization of Bio-Resources,Yunnan University,Kunming 650091,China,2.Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,3.Yunnan Academy of Tobacco Agricultural Science,Yuxi 653100,4.Yunnan Yunye Fertilizer Co.,Ltd,Kunming 650217,5.Shandong TaiFengYuan Biotechnology Co.,LTD,Jinan 250014,China) |
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| Abstract: | A total of 165 isolates with antagonistic effects against Phytophthora parasitica var.nicotianae were screened from 970 endophytic bacteria isolated from health leaves of tobacco variety NC297.Bioassay results in vivo showed that these antagonists exhibited different inhibitory activities to the pathogen which ranged from 9.55% to 55.96%.The biomass of endophytic bacteria varied not significantly during periods of seeding to maturity but the amount of antagonists increased in the growth process of tobacco.T... |
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| Keywords: | NC297 endophytic bacteria biological control Phytophthora parasitica var nicotianae phylogenetic analysis |
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