| "Anti-UV Clone" 柴胡细胞提取物对UV-B照射HaCaT细胞的影响 |
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| 引用本文： | 李育中,范静,沈霏,王建光,杨菊芬,吕琦,马海英,夏光敏,陈穗云. "Anti-UV Clone" 柴胡细胞提取物对UV-B照射HaCaT细胞的影响[J]. 云南大学学报(自然科学版), 2011, 33(4): 469-474 |
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| 作者姓名： | 李育中 范静 沈霏 王建光 杨菊芬 吕琦 马海英 夏光敏 陈穗云 |
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| 作者单位： | 云南大学生命科学学院;中国医学科学院北京协和医学院医学生物学研究所;山东大学生命科学学院; |
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| 基金项目： | 国家自然科学基金资助项目(30760089) |
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| 摘 要： | 利用噻唑蓝比色分析法(MTT法)研究"Anti-UV Clone"柴胡细胞株不同溶剂提取物对UV-B照射HaCaT细胞活性的影响.实验结果表明,"Anti-UV Clone"柴胡细胞株乙醇和乙酸乙酯提取物均抑制HaCaT细胞的活性,而水提取物则能提高HaCaT细胞的活性.因此,"Anti-UV Clone"柴胡细胞株水提取物进一步被用于HaCaT细胞紫外线照射研究.与UV-B照射组相比,在照射前或照射后加入"Anti-UV Clone"柴胡细胞株水提取物均能不同程度提高细胞的活性(p<0.05);在照射前或照射后加入0.2U/mL的"Anti-UV Clone"柴胡细胞株水提取物,细胞活性分别比照射组提高10.54%或14.95%.说明"Anti-UV Clone" 柴胡细胞株水提取物中可能含有UV-B保护物质.
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| 关 键 词： | 南柴胡 提取物 UV-B HaCaT细胞 MTT |
| 收稿时间： | 2010-11-30 |
Effects of Anti-UV Clone extracts on cell viability of HaCaT cells exposure to UV-B |
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| LI Yu-zhong,FAN Jing,SHEN Fei,,WANG Jian-guang,YANG Ju-fen,LV Qi,MA Hai-ying,XIA Guang-min,CHEN Sui-yun. Effects of Anti-UV Clone extracts on cell viability of HaCaT cells exposure to UV-B[J]. Journal of Yunnan University(Natural Sciences), 2011, 33(4): 469-474 |
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| Authors: | LI Yu-zhong FAN Jing SHEN Fei WANG Jian-guang YANG Ju-fen LV Qi MA Hai-ying XIA Guang-min CHEN Sui-yun |
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| Affiliation: | LI Yu-zhong1,FAN Jing1,SHEN Fei1,2,WANG Jian-guang1,YANG Ju-fen1,LV Qi1,MA Hai-ying1,XIA Guang-min3,CHEN Sui-yun1(1.School of Life Sciences,Yunnan University,Kunming 650091,China,2.Institute of Medical Biology,Chinese Academy of Medical Sciences & Peking Union Medical College,Kunming 650118,3.School of Life Sciences,Shandong University,Jinan 250100,China) |
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| Abstract: | In this study,MTT assay was carried out to study the effect of different extracts from Anti-UV Clone(Obtained from Bupleurum scorzonerifolium Willd.callus) on the viability of human keratinocyte cells(HaCaT cells) exposure to UV-B radiation.The results showed that both ethanol and ethyl acetates extract of the cell clone inhibited the viability of HaCaT cells.However the viability of HaCaT cells was promoted by the water extract of the cell clone in a dose dependent manner,thus the water extract was chose t... |
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| Keywords: | Bupleurum scorzonerifolium Willd extract UV-B HaCaT cell MTT |
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