气液色谱填充柱双抽涂渍法

对一种新的气液色谱固定相制备形式--抽真空抽滤涂渍法进行了初步研究.以4种正构烷烃的同系物为测试样品,在相同的操作条件下,此方法与抽空蒸发法(固定液与载体质量配比≤3%)和常规法(配比≥5%)比较分析周期、理论塔板数、分离度、拖尾因子等各色谱参数.结果显示:在分离度,拖尾因子基本相同的情况下,固定液与载体质量配比为1%,2%,3%时,抽真空抽滤涂渍法制备的色谱柱比抽空蒸发法制备的色谱柱分析周期分别缩短10%,20%,26%,理论塔板高度分别降低12%,5%,12%;在配比为5%,7%时,分析周期和理论塔板数无显著性差异.

Double Suction Straining Coating Method for the Preparation of Packed Column of Gas-liquid Chromatography

A new procedure which is called double suction straining coating method for the preparation of gas-liquid chromatographic stationary phase was reported.The result of the study showed that at the percentage(mass of liquid phase/mass of support)of 1%,2% and 3%,the analytical speed and the HETP(height equivalent to theoretical plate)of the column prepared by the double suction straining coating method are always higher than those prepared by the suction and evaporation method.At the percentage of 1%,2% and 3%,the analytical speed of the two columns prepared by the new coating method is 10%,20%and 26% faster than the columns prepared by the compared coating method respectively and HETPs of the two new columns are 12%,5%,12% lower than the compared columns.At the percentage of 5 % and 7%,there are no apparent difference between the two compared methods. The chromatographic condition is H2 Carrier gas,6201 red support(60～80 mesh),Apiezon-L stationary phase,stainless steel column(1 m×3 mm i.d.),TCD detector and the mixture of test sample of n-hexane,n-heptane,n-octane and n-nonane.