泰山四叶参多糖的提取、含量测定及抗氧化活性研究

本实验研究泰山四叶参多糖的提取、分离、纯化方法,并测其含量,探讨其抗氧化能力.方法是建立热水提取、乙醇沉淀、Sevage法除蛋白及透析、纯化泰山四叶参多糖的方法,采用苯酚-浓硫酸法测定其含量,并比较了不同浓度的四叶参多糖（200、400、600、800、100μg/ml）和抗坏血酸（Vc50、100、200、400、600、800、100μg/ml）的还原能力以及不同浓度的泰山四叶参多糖和抗坏血酸（均为40、80、160、320、600、800μg/ml）清除二苯代苦味酰基（DPPH）自由基的能力.结果显示,本实验提取得到泰山四叶参多糖含量为11.09%,泰山四叶参多糖的还原能力及清除DPPH自由基的能力随其浓度的增加而增大,在800μg/ml时,DPPH自由基清除率达到90%以上,接近抗坏血酸的清除率.因此,泰山四叶参多糖具有较好的抗氧化能力.

Research on the Extraction,Determination and Antioxidant Capacity of Polysaccharides from Radix Codonopsis Lanceolatae from Mount Taishan

Objective： To research the method of the extraction,determination and antioxidant capacity of Polysaccharides from Radix Codonopsis Lanceolatae（RCLP） from Mount Taishan.Methods： Polysaccharides was extracted with hot water,subsided with 95% ethanol,separated with protein with Sevage assay and then dialyzed and purified.The content of RCLP was assayed with phenol vitriolic colorimetry.We determinate and compare the deoxidization activities of RCLP（200,400,600,800,1000μg/ml） and vitamin C（50,100,200,400,600,800,1000μg/ml） and clearing DPPH free radical activities of RCLP and vitamin C（40,80,160,320,600,800μg/ml）.Result： With augmentation of concentration,the deoxidization and scavenging DPPH free radical capacity of RCLP increased continuously.RCLP（800μg/ml） could clear 90% DPPH free radical.That was close to Vc.Conclusion： RCLP from Mount Taishan has relatively strong antioxidant activity.