| 去污剂／聚合物双水相体系在膜蛋白分离中的应用 |
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| 引用本文： | 刘娟,朱建航,范杰平. 去污剂／聚合物双水相体系在膜蛋白分离中的应用[J]. 江西科学, 2008, 26(3): 416-420 |
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| 作者姓名： | 刘娟 朱建航 范杰平 |
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| 作者单位： | 南昌大学化学工程系,江西南昌,330031 |
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| 摘 要： | 去污剂／聚合物双水相体系主要依靠胶束和聚合物分子间作用力来实现成相，具有对疏水膜蛋白增溶作用、成相物浓度低、萃取环境温和及操作简单等特点，可以克服传统膜蛋白提取工艺的一些缺陷；工艺开发过程中疏水膜蛋白以疏水作用富集于去污剂相，亲水蛋白以体积排阻效应富集于聚合物相，从而实现对膜蛋白的分离纯化。
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| 关 键 词： | 去污剂 膜蛋白 胶束 双水相 |
Application of Detergent/polymer Aqueous Two Phase Systems for Purification of Membrane Protein |
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| LIU Juan,ZHU Jian-hang,FAN Jie-ping. Application of Detergent/polymer Aqueous Two Phase Systems for Purification of Membrane Protein[J]. Jiangxi Science, 2008, 26(3): 416-420 |
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| Authors: | LIU Juan ZHU Jian-hang FAN Jie-ping |
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| Affiliation: | ( Department of Chemical Engineering, Nanchang University, Jiangxi Nanchang 330031 PRC) |
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| Abstract: | Detergent/polymer aqueous two-phase systems(ATPS)could improve the efficiency of membrane protein extraction,due to its advantages as follows:(1)extraction of membrane proteins in the detergent-rich phase;(2)low concentration of the phase-formed substances;(3)the mild and convenient extraction conditions.Membrane proteins could be selectively partitioned into the micelle phase while hydrophilic proteins to the polymer phase to achieve its purification. |
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| Keywords: | Detergent Membrane protein Micelle Aqueous two-phase systems |
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