| 斜带石斑鱼神经坏死病毒主衣壳蛋白的原核表达与纯化 |
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| 引用本文： | 陈晓艳,翁少萍,殷志新,何建国,HE Jian-guo.斜带石斑鱼神经坏死病毒主衣壳蛋白的原核表达与纯化[J].中山大学学报(自然科学版),2005,44(6):83-86. |
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| 作者姓名： | 陈晓艳 翁少萍 殷志新 何建国 HE Jian-guo |
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| 作者单位： | 1. 中山大学生命科学学院,广东,广州,510275;暨南大学生物工程学系,广东,广州,510632
2. 中山大学生命科学学院,广东,广州,510275 |
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| 基金项目： | 国家科技攻关项目，广东省自然科学基金团队项目，广东省科技攻关项目 |
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| 摘 要： | 将含有斜带石斑鱼Epinephelus coioides神经坏死病毒(orange-spotted grouper nervous necrosis virus,OGNNV)主衣壳蛋白(major capsid protein, MCP)基因的重组表达质粒载体pET32a-MCP转化到大肠杆菌BL21(DE3)中进行融合表达,经SDS-PAGE分析和Western-blot鉴定,证实了重组大肠杆菌融合表达了斜带石斑鱼神经坏死病毒主衣壳蛋白.表达的融合蛋白主要以不可溶的包涵体形式存在,提取的包涵体中融合蛋白含量占60%以上,经柱层析纯化蛋白,纯化度达90%以上.
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| 关 键 词： | 斜带石斑鱼Epinephelus coioides 神经坏死病毒 主衣壳蛋白 原核表达 |
| 文章编号： | 0529-6579(2005)06-0083-04 |
| 收稿时间： | 12 8 2004 12:00AM |
| 修稿时间： | 2004年12月8日 |
Expression and Purification of Major Capsid Protein of Orange-spotted Grouper Nervous Necrosis Virus |
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| CHEN Xiao-yan,WENG Shao-ping,YIN Zhi-xin,ZHANG Min,HE Jian-guo.Expression and Purification of Major Capsid Protein of Orange-spotted Grouper Nervous Necrosis Virus[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2005,44(6):83-86. |
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| Authors: | CHEN Xiao-yan WENG Shao-ping YIN Zhi-xin ZHANG Min HE Jian-guo |
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| Institution: | 1. School of Life Sciences, Zhongshan University, Guangzhou 510275, China; 2. Department of Biotechnology, Jinan University, Guangzhou 510632, China |
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| Abstract: | Fish nodaviruses,members of the Nodaviridae family,are the causative agents of viral nervous necrosis(VNN) in many fishes.In this study,recombant expression vector pET32a-MCP which inserted with major capsid protein gene(MCP) of orange-spotted nervous necrosis virus(OGNNV) is transformed into BL21.SDS-PAGE and Western blot analysis confirm that the transformed E.coli BL21 can express MCP protein fused with polytide.Fusion-proteins are produced as inclusion bodies in BL21.The recombinant proteins are purified with above 90% purity level. |
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| Keywords: | Epinephelus coioids nervous necrosis virus major capsid protein prokaryotic expression |
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