金花小檗功效成分及其抑菌和抗氧化活性的研究

为充分利用川西南民族药——金花小檗,用不同的方法对其抑菌及抗氧化活性进行了测定.用酸性染料比色法、 HPLC法测定了金花小檗根、茎、醇提物、萃取部位的功效组分含量,发现生物碱集中于正丁醇部位和水部位中,小檗碱在醇提物、萃取部位中的含量都最多,巴马汀最少.测定其根、茎、醇提物对金黄色葡萄球菌、大肠杆菌的抑菌作用可知:与75%乙醇相比,金花小檗根、茎、醇提物对金黄色葡萄球菌的抑菌效果为低敏;而与75%乙醇相比,金花小檗根、茎、醇提物对大肠杆菌的抑菌效果为中敏.用DPPH·清除率法、总还原力法测定其醇提物和萃取部位的抗氧化活性,结果相似:正丁醇部位、水相部位的抗氧化活性较强,石油醚萃取部位的抗氧化活性最低.用金花小檗醇提物、正丁醇-水部位分别饲喂小鼠30 d测定小鼠血清MDA和T-SOD值,所得结果与正常组比较,试验组小鼠T-SOD值显著降低, MDA值显著升高,说明金花小檗有显著的抗氧化活性.因此,金花小檗提取物、萃取部位不仅有显著的抑菌活性,而且也有较高的体、内外抗氧化活性.

On Effect of Berberis wilsonae Functional Components and Antimicrobial and Antioxidant Activities

In order to make full use of southwest minority medicinal plant, Berberis wilsonae, in Sichuan Province, its extracts and fragments were tested for antibacterial and antioxidant activity. It was determined by the acidic dye spectrophotometry or HPLC for the function composition in B. wilsonae root and stem, extracts and fragments. The alkaloids were mainly in the 1-butanol segment and water segments. Berberine was most rich in the alcohol extracts but Bamatin was least. Compared with 75% alcohol, the inhibitory effects of B. wilsonae rhizome and the extract on Staphylococcus aureus were significantly inhibitedand antibacterial effect was low sensitivity. Compared with 75% alcohol, the inhibitory effects of B. wilsonae rhizome and the extract on the E. Coli were extremely remarkable and antibacterial effect was medium sensitivity. The antioxidation activity of the B. wilsonae extracts and fragments was determined by DPPH and total reduction method. The results obtained by the two methods were similar. It shows that the anti-oxidative activity of 1-butanol and water fragment was stronger, and the antioxidant activity of petroleum ether fragment was the lowest. The mice serum MDA and T-SOD value were determined by using B. wilsonae extracts and 1-butanol-water segment respectively to feed mice 30 d. The result of the experiment was the case that compared with the normal group, the experimental group T-SOD value decreased significantlyand the MDA level increased significantly. This test shows that the extract treatment could substantially improve the antioxidant activity of mice. Therefore, B. wilsonae extract, fragments have significant inhibitory activity, but also has high antioxidant activity, both in vivo and in vitro.