Clonogenic growth of acute non-lymphocytic leukemia cells in serum-free medium |
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Authors: | P A Bernabei V Santini O Dal Pozzo R Bezzini V Gattei R Saccardi G Rombolà P R Ferrini |
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Affiliation: | Università degli Studi di Firenze, Policlinico di Careggi, Italy. |
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Abstract: | We devised a serum-free medium for growth of leukemic colony-forming units (CFU-L), enriched with albumin, transferrin, lipids, insulin, hydrocortisone and oligoelements. Blast cells from 15 patients affected by acute non-lymphocytic leukemia were grown in this medium in the presence of human placental conditioned medium obtained under serum-free conditions (sfHPCM). Their clonogenic growth was comparable with that obtained in a serum-containing system. Furthermore, when serum-free cultures were carried out in absence of sfHPCM, either CFU-L growth was prevented or, if clones were obtained, the cultures showed a marked decrease in clonogenicity, indicating their strict dependence on growth factors. |
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