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Mapping of restriction enzyme cuts by a new two-dimensional procedure |
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| Authors: | C W Chen R Braun C A Thomas Jr |
| Institution: | (1) Department of Cellular Biology, Scripps Clinic and Research Foundation, 10666 N. Torrey Pines Rd, 92037 La Jolla, California, USA;(2) Present address: Panlabs Fermtech Division, Panlabs Taiwan Ltd, P.O. Box 26-127, Taipei, Taiwan;(3) Present address: Institute of General Microbiology, University of Berne, Baltzer-Strasse 4, CH-3012 Bern, Switzerland;(4) Present address: Helicon Foundation, 11095 Toreyana Rd, 92037 San Diego, CA, USA |
| Abstract: | Summary A new procedure has been worked out to establish restriction maps. The method is fast, does not in general require labeled DNA and has been applied to map the linear palindromic rDNA ofPhysarum with the restriction enzymeBstEII.Acknowledgment. This work was supported by grants from the NIH (GM-30338), the NSF (PCM 8116391) and the Swiss National Science Foundation (3.075.81). |
| Keywords: | Physarum ribosomal DNA restriction method two-dimensional gel electrophoresis |
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