| 芸芥发状根的诱导及其植株再生体系的建立 |
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| 引用本文： | 武振华,牛炳韬,张涛,祁永和,王新宇. 芸芥发状根的诱导及其植株再生体系的建立[J]. 兰州大学学报(自然科学版), 2007, 43(5): 44-47,53 |
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| 作者姓名： | 武振华 牛炳韬 张涛 祁永和 王新宇 |
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| 作者单位： | 兰州大学,生命科学学院,甘肃,兰州,730000;中国科学院,近代物理研究所,甘肃,兰州,730000;兰州大学,生命科学学院,甘肃,兰州,730000 |
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| 摘 要： | 通过发根农杆菌R1000介导的遗传转化建立了芸芥发状根的诱导、培养及植株再生体系.研究了影响发状根诱导及生长的各种因素,通过PCR对所得发状根进行了分子水平的鉴定.结果表明:发根农杆菌R1000的rol B基因已被成功地转入并整合到该发根培养物的基因组中.
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| 关 键 词： | 芸芥 发状根 发根农杆菌 |
| 文章编号： | 0455-2059（2007）05-0044-05 |
| 修稿时间： | 2006-12-062007-09-14 |
Hairy roots induction and plant regeneration in Eruca sativa Mill |
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| WU Zhen-hua,NIU Bing-tao,ZHANG Tao,QI Yong-he,WANG Xin-yu. Hairy roots induction and plant regeneration in Eruca sativa Mill[J]. Journal of Lanzhou University(Natural Science), 2007, 43(5): 44-47,53 |
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| Authors: | WU Zhen-hua NIU Bing-tao ZHANG Tao QI Yong-he WANG Xin-yu |
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| Affiliation: | 1. School of Life Sciences, Lanzhou University, Lanzhou 730000 China; 2. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China |
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| Abstract: | The system of induction and culture of hairy roots derived from cotyledon mediated by Agrobac- terium rhizogenes R1000 and the plant regeneration from hairy roots in Eruca sativa Mill were established, and the factors that affect the inducement and growth of hairy roots were also studied. Furthermore, this study testified the hairy roots in molecular level by the PCR analysis. The results of such identification demonstrated that rol B gene of T-DNA from Ri plasmid had been integrated into the genome of the transformed roots. |
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| Keywords: | Eruca sativa Mill hairy root agrobacterium rhizogene |
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