响应面法优化洋葱伯克霍尔德菌固定化脂肪酶催化合成生物柴油工艺

以自制的洋葱伯克霍尔德菌固定化脂肪酶为催化剂，在微水相、无溶剂体系中研究了大豆油和甲醇合成生物柴油的工艺。在系统考察了酶用量、醇油比、含水量、反应温度、反应时间、甲醇流加方式等因素对甲酯得率影响的基础上，利用响应面试验设计优化了各主效因子，建立甲酯得率的二次回归方程，获得了最优的工艺条件：加酶量2.4%、加水量7.1%、反应温度40.4℃、反应时间10.7h、 醇油比4.5。在此条件下，实验测得甲酯得率为97.2%，与响应面模型预测值96.9%非常吻合，说明该优化方法有效、可靠。

Optimization of the production of biodiesel catalyzed by immobilized lipase from Burkholderia cepacia using a response surface method

Lipase from Burkholderia cepacia has been immobilized and used for the production of biodiesel from soybean oil and methanol in a microaqueous and solvent-free system. The effects of the enzyme loading, water content, reaction temperature, reaction time, methanol/oil molar ratio and number of times of adding methanol on the yield of methyl ester were investigated in detail, and the factors having a major impact were optimized by a response surface method. A quadratic regression equation for the yield of methyl ester was then established. The results showed that the optimum conditions were an enzyme loading of 2.4%, water content of 7.1%, reaction temperature of 40.4℃, reaction time of 10.7 h, and methanol/oil molar ratio of 4.5. Under these conditions, the measured yield of methyl ester was 97.2%, which was very close to the predicted value of 96.9%. This indicates that the optimization method is effective and reliable.