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稳定剂对糖化酶溶液的保护作用
引用本文:张贺迎,武金霞,张瑞英,宋春凤. 稳定剂对糖化酶溶液的保护作用[J]. 河北大学学报(自然科学版), 2002, 22(4): 374-376. DOI: 10.3969/j.issn.1000-1565.2002.04.016
作者姓名:张贺迎  武金霞  张瑞英  宋春凤
作者单位:河北大学,生命科学学院,河北,保定,071002
摘    要:用大分子亲水性多糖黄原胶、动物蛋白明胶、无机盐类--氯化钠、硝酸钠、磷酸氢二钠及甘油分别处理糖化酶溶液,可明显提高糖化酶的稳定性,含有3 g/L黄原胶、10 mmol/L氯化钠和90 g/L甘油的复合稳定剂和1 g/L山梨酸钾的酶液,室温下保存60 d,其酶活保留率达89.6%,较对照高出28.4%.

关 键 词:糖化酶  稳定剂  防腐剂  热稳定性  
文章编号:1000-1565(2002)04-0374-03
修稿时间:2002-01-19

Protective Effect of Stabilizer on Glucoamylase Solution
ZHANG He-ying,WU Jin-xia,ZHANG Rui-ying,SONG Chun-feng. Protective Effect of Stabilizer on Glucoamylase Solution[J]. Journal of Hebei University (Natural Science Edition), 2002, 22(4): 374-376. DOI: 10.3969/j.issn.1000-1565.2002.04.016
Authors:ZHANG He-ying  WU Jin-xia  ZHANG Rui-ying  SONG Chun-feng
Abstract:Macromolecular hydrophilic polysaccharide xanthan gum ,animal protein geltin casein,mineral salt(sodium chloride, sodium nitrate, disodium hydrogen phosphate),glycerin and some preservatives can greatly improve the thermostability of glucoamylase. A stabilizer complex containing 3 g/L xanthan gum,10 mmol/L NaCl,90 g/L glycerin and 1 g/L potassium sorbate has great protective effect on glucoamylase. After storing at room temperature for 60 days, the remaining glucoamylase activity is 89.6% which is higher 28.4% than control where no stabilizer was present.
Keywords:glucoamylase  stabilizer  preservative  thermostability
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