Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport |
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Authors: | R Diaz L S Mayorga P J Weidman J E Rothman P D Stahl |
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Institution: | Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110. |
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Abstract: | In reconstitution studies N-ethylmaleimide, a sulphydryl alkylating reagent, inhibits both fusion of endocytic vesicles and vesicular transport in the Golgi apparatus. We show here that the same N-ethylmaleimide-sensitive factor that catalyses the vesicle-mediated transport within Golgi stacks is also required for endocytic vesicle fusion. Thus, it is likely that a common mechanism for vesicle fusion exists for both the secretory and endocytic pathways of eukaryotic cells. |
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