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Non-viral gene carrier mediated short hairpin RNA interference for inhibition of tumor cells growth |
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| Authors: | YaJun Duan CuiHong Yang ZhenFang Zhang JianFeng Liu JunNian Zheng Yong Xu DeLing Kong YaoTing Yu |
| Affiliation: | 1 Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, China; 2 Laboratory of Urology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China; 3 Department of Urology, Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin 300211, China |
| Abstract: | A tumor-targeting gene vector G250mAb-PEI-PEG has been prepared by modification of polyethylenimine (PEI) with polyethyleneglycol (PEG) and G250, a monoclonal antibody against the G250 antigen on tumor cell surface. The transfection efficiency was as high as 70% in G250 positive HeLa cells, whereas the transfection efficiency was relatively low (30%) in normal NIH3T3 cells. A plasmid encoding the short hairpin RNA (shRNA) specific for nucleostemin gene (NS) was efficiently transfected into the HeLa cells wi... |
| Keywords: | nucleostemin nonviral gene vectors gene therapy RNA interference targeted gene therapy |
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