首页 | 本学科首页   官方微博 | 高级检索  
     

酶法生产鸟氨酸菌株的筛选与培养
引用本文:张鹏,徐焘,张骁,陈畅. 酶法生产鸟氨酸菌株的筛选与培养[J]. 北京化工大学学报(自然科学版), 2009, 36(6): 85-90
作者姓名:张鹏  徐焘  张骁  陈畅
作者单位:北京化工大学,生命科学与技术学院,北京,100029;北京化工大学,生命科学与技术学院,北京,100029;北京化工大学,生命科学与技术学院,北京,100029;北京化工大学,生命科学与技术学院,北京,100029
基金项目:北京化工大学青年教师自然科学基金 
摘    要:从土壤中筛选到一株可以利用胞内精氨酸酶生产鸟氨酸的菌株XT-025,通过形态特征、生理生化特性及16S rRNA基因序列分析,初步鉴定为苏云金芽孢杆菌(Bacillus thuringiensis),这是首次发现该种微生物可用以生产鸟氨酸。通过对其培养条件和转化条件的优化,确定了最佳的培养时间为24h,最合适的转化液成分为0.3mol/L的碳酸盐缓冲液,V(Na2CO3)∶V(NaHCO3)为8∶2。同时发现Mn2+对精氨酸酶有很强激活作用,在添加适量Mn2+的条件下,鸟氨酸的产量能够达到50g/L,精氨酸摩尔转化率达到100%,而Sn4+和Cu2+则会使酶完全失活。

关 键 词:鸟氨酸  精氨酸  精氨酸酶  苏云金芽孢杆菌
收稿时间:2009-03-02

Screening of bacterium for the conversion of arginine into ornithine by arginase
ZHANG Peng,XU Tao,ZHANG Xiao,CHEN Chang. Screening of bacterium for the conversion of arginine into ornithine by arginase[J]. Journal of Beijing University of Chemical Technology, 2009, 36(6): 85-90
Authors:ZHANG Peng  XU Tao  ZHANG Xiao  CHEN Chang
Affiliation:College of Life Science and Technology, Beijing University of Chemical T echnology, Beijing 100029, China
Abstract:A strain of bacteria XT-025 for producing ornithine by arginase was screened from soil. This strain was identified as Bacillus thuringiensis according to its morphology, physiological characteristics and 16S ribo-somal RNA gene sequence. This is the first report of the production of ornithine by this microbe. The optimum culture time was found to be 24 h with a cushion solution composed of 0.3mol/L carbonate and V(Na_2CO_3): V (NaHCO_3) =8 = 2. It was found that Mn~(2+) can activate arginase and by adding appropriate concentrations of Mn~(2+), the yield of ornithine can reach 50 g/L and the molar conversion rate of arginine can reach 100%. In contrast, Sn~(4+) and Cu~(2+) were found to inhibit the reaction.
Keywords:ornithine  arginine  arginase  Bacillus thuringiensis  
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《北京化工大学学报(自然科学版)》浏览原始摘要信息
点击此处可从《北京化工大学学报(自然科学版)》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号