| 利福平对大肠杆菌F′质粒的消除 |
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| 引用本文： | 李林双.利福平对大肠杆菌F′质粒的消除[J].西南师范大学学报(自然科学版),1990(3). |
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| 作者姓名： | 李林双 |
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| 作者单位： | 西南师范大学生物系 |
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| 摘 要： | 研究了利福平(rif)对大肠杆菌F′质粒的消除效应.实验结果表明,消除质粒以rif的浓度50—100μg/mL为宜.通过选择培养,在LB和LB+cm平板上,就能选出F~-菌株,因F~+抗cm,E~-不抗cm.选得的F~-菌株是:E.Coli XAC F~-/△(lac pro)nal.
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| 关 键 词： | 大肠杆菌 质粒 消除 利福平 |
STUDIES ON THE F' PLASMIDS CURING OF E. COLI STRAINS WITH RIFAMPIN |
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| Li Linshuang.STUDIES ON THE F'''' PLASMIDS CURING OF E. COLI STRAINS WITH RIFAMPIN[J].Journal of Southwest China Normal University(Natural Science),1990(3). |
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| Authors: | Li Linshuang |
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| Institution: | Southwest China Teachers University |
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| Abstract: | This paper reports an efficient method of F' plasmids curing with rifampin. The replication of plasmids can be selectively inhibited by rifampin. Crowth of strains carrying F or F' factors in rifampin therefore results in loss of the sex factor. The result shows that the concentrations of rifampin of 50-100ng/mL are widely used for this purpose. In this method, only by using two selectivity medium can the strains of F+ or F- be selected. Thus, the selection is for E.coli XAC F-/A (lar pro) nal. |
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| Keywords: | escherichia coli plasmid curing rifampin |
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