| 苋菜红-蛋白质体系的共振瑞利散射光谱及其应用 |
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| 引用本文： | 金俊成,常文贵,高云.苋菜红-蛋白质体系的共振瑞利散射光谱及其应用[J].皖西学院学报,2012,28(5):106-109. |
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| 作者姓名： | 金俊成 常文贵 高云 |
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| 作者单位： | 皖西学院材料与化工学院,安徽六安237012 安徽仿生传感与检测技术省级实验室,安徽六安237012 |
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| 摘 要： | 研究了酸性条件下染色剂苋菜红-蛋白质体系的共振瑞利散射光谱。在pH=3．80的Clark-Lubs缓冲介质中，苋菜红与蛋白质通过分子间作用力形成复合物，使最大波长为523nm的共振光散射光谱得到加强，这种增强的共振光散射用于鸡蛋清试样中总蛋白的测定，其线性响应范围为1．5～5．0mg／L，检测限为209μg／L。该方法的稳定性及选择性良好，适用于微量蛋白质分析。
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| 关 键 词： | 共振瑞利散射光谱 苋菜红 蛋白质 |
Study on the Resonance Light Scattering and Analytical Application of Protein-Amaranth System |
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| JIN Jun-cheng,CHANG Wen-gui,GAO Yun.Study on the Resonance Light Scattering and Analytical Application of Protein-Amaranth System[J].Journal of Wanxi University,2012,28(5):106-109. |
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| Authors: | JIN Jun-cheng CHANG Wen-gui GAO Yun |
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| Institution: | 1,2(1.Department of Materials and Chemical Engineering,West Anhui University,Lu’an 237012 China; 2.Key Laboratory of Biomimetic Sensor and Detection Technology in Anhui,Lu’an 237012,China) |
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| Abstract: | Resonance light scattering （RLS） spectrum of Amaranth -Protein system was studied in this paper. In Clark-Lubs buffer medium of pH 3.80, the Amaranth-Protein composites formed via intermolecular forces, which enhancing the resonance light scattering at maximum wavelength of about 523 nm. Based on the enhancement of RLS, a novel method for the determination of proteins was developed. The line arrange for the determination of protein is 1.5 to 5.0 mg/L, limit of detection is 209μg/L. The method is stability and selectivity, which can be used to determine trace protein. |
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| Keywords: | Resonance Light Scattering Amaranth protein |
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