大别山区产葛根黄酮对CCl4诱导的小鼠化学性肝损伤保护作用

研究大别山区葛根总黄酮（TPF）对CCl4诱导小鼠化学性肝损伤的保护作用及可能的作用机制。采用1次性腹腔注射（ip）0．1％CCl4花生油溶液（10ml／kg）建立小鼠化学性肝损伤模型。TPF连续灌胃（ig）14d后摘眼球取血，分离血清，测定血清中丙氨酸氨基转移酶（ALT）和天门冬氨酸氨基转移酶（AST）活性；剖腹取肝，制备10％肝匀浆，测定肝组织中超氧化物歧化酶（SOD）、丙二醛（MDA）、干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）活性；进行肝组织病理切片，观察肝组织病理学变化。结果显示TPF能降低CCl4诱导的化学性肝损伤小鼠血清中ALT、AST和肝匀浆中MDA、IFI-γ、TNF-α的活性及增强肝匀浆中SOD的活力。其作用机制可能与抗氧化和降低肝组织中IFN-γ、TNF-α水平有关。

Protective Effects of TPF from Dabie Mountains on Chemical Liver Injury in Mice Induced by CCl4

The protective effects and the mechanisms were studied about TPF from Dabie Mountains on chemical liver injury in mice induced by CCl4. The mouse was Injected 0. 1% CCl4 solution dissolved in peanut oil （10 ml/kg） into it＇s abdominal cavity to set animal models of chemical liver injury. The blood samples were drawn out to determine ALT and AST by intragastric administration with TPF for 14d. The liver tissue homogenates were drawn for the determination of IFN-γ/and TNF-α. And the liver histopathology was studied with morphological method. The results showed that, compared with model group, TPF could decrease ALT, AST in serum and MDA, IFN-γ, TNF-α in liver, and increase the activity of SOD in liver. TPF could protect against chemical liver injury induced by CCl4 in mice. The mechanisms may be related to the antioxidant activity of TPF and it＇s effect to decrease the IFN-γ and TNF-α level in liver.