| 芝麻油中芝麻素提取与纯化工艺研究 |
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| 引用本文： | 何晓梅,朱珣.芝麻油中芝麻素提取与纯化工艺研究[J].皖西学院学报,2012,28(5):9-11,15. |
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| 作者姓名： | 何晓梅 朱珣 |
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| 作者单位： | 皖西学院生物与制药工程学院,安微六安237012 安徽省植物生物技术实训中心,安徽六安237012 |
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| 基金项目： | 基金项目:六安市定向委托研究项目，皖西学院抗体制备及其质量检测中心资助 |
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| 摘 要： | 以芝麻油为原料，用乙醇作提取剂，通过单因素试验和正交试验确定芝麻素最佳提取条件。采用乙醇对芝麻素粗品进行结晶纯化，测定其熔点，并利用高效液相色谱法测定其纯度。结果表明料液比为1：5，浸提时间3h，温度50℃，浸提3次，芝麻素提取率最高，达到0．336g／100g芝麻油。芝麻素结晶熔点为120．5～121．7℃，HPLC法分析芝麻素物质相对含量达95．2％。本研究得到较高纯度的芝麻素纯化结晶，为开发高附加值的芝麻素产品奠定了良好的基础。
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| 关 键 词： | 芝麻素 熔点 HPLC |
Studies on Extraction and Purification Technology on Sesamin. a Bioactive Substance |
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| HE Xiao-mei,ZHU.Studies on Extraction and Purification Technology on Sesamin. a Bioactive Substance[J].Journal of Wanxi University,2012,28(5):9-11,15. |
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| Authors: | HE Xiao-mei ZHU |
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| Institution: | 1,2(1.College of Biology and Pharmaceutical Engineering,West Anhui University,Lu’an 237012,China; 2.Anhui Plant Biotechnology Training Centre,Lu’an 237012,China) |
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| Abstract: | Alcohol as extraction solvent, the optimum condition of extraction of sesamin from sesame oil was obtained by single factor tests and orthogonal test. Then the crude extract sesamin was purified with alcohol solvent, determining the sesamin melting temperature and analyzing by HPLC. The result showed that the optimum conditions for the extraction are as followings： solid- liquid ratio 1 ： 5, extraction time 3 h, temperature 50 ℃, extracting times 3. The crude extract accounted for 0. 336 g/100 g from sesamin oil. The crude extract was further purified by crystallization technology. The melting range of the purified sesamin is 120. 5-121.7 ℃. The sesamin content of the purified sesamin reaches 95.2% by HPLC analysis. High purified sesamin was prepared by this work. It would he help to developing high additional value sesamin products. |
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| Keywords: | sesamin melting temperature HPLC |
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