| 甘蔗黑穗病菌拮抗性芽孢杆菌的抗菌作用与伊枯草菌素A的产生有关 |
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| 引用本文： | 张桂英,廖咏梅,张君成. 甘蔗黑穗病菌拮抗性芽孢杆菌的抗菌作用与伊枯草菌素A的产生有关[J]. 广西科学, 2004, 11(3): 269-272 |
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| 作者姓名： | 张桂英 廖咏梅 张君成 |
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| 作者单位： | 广西大学农学院,南宁市大学路100号,530004;广西大学农学院,南宁市大学路100号,530004;广西大学农学院,南宁市大学路100号,530004 |
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| 基金项目： | 广西自然科学基金资助项目 (桂科自 9912 0 15) |
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| 摘 要： | 选择对甘蔗黑穗病菌有拮抗作用的5个革兰氏阳性芽孢杆菌拮抗菌株和3个革兰氏阴性拮抗细菌菌株，用PCR技术，扩增抗霉菌枯草杆菌素操纵元中mycB基因。结果是，5个革兰氏阳性芽孢杆菌拮抗菌株均扩增出一条大小为2.0kb左右的特异带，革兰氏阳性芽孢杆菌基因组的PCR产物和伊枯草菌素A合成酶操纵元中的，ItuB基因的同源性为97％～98％，说明其抗菌作用机制和Iturin类群脂肽抗生素的产生有关。3个革兰氏阴性拮抗细菌中，没有扩增到基因，其抗菌作用机制有待进一步研究。
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| 关 键 词： | 甘蔗黑穗病菌 拮抗细菌 伊枯草菌素A |
| 收稿时间： | 2004-03-31 |
| 修稿时间： | 2004-03-31 |
The Antifungal Mechanism of Antagonistic Bacillus subtilus to Ustilago scitaminea is Related to Iturin A Producing |
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| Zhang Guiying,Liao Yongmei and Zhang Juncheng. The Antifungal Mechanism of Antagonistic Bacillus subtilus to Ustilago scitaminea is Related to Iturin A Producing[J]. Guangxi Sciences, 2004, 11(3): 269-272 |
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| Authors: | Zhang Guiying Liao Yongmei Zhang Juncheng |
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| Affiliation: | Zhang Guiying Liao Yongmei ** Zhang Juncheng |
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| Abstract: | gram-positive Bacillus strains and 3 gram-negative bacteria strains which are antagonistic to Ustilago scitaminea were selected to be amplified the mycB gene of iturin A operon by PCR.A band of about 2.0 kb appeared among all strains of 5 gram-positive Bacillus.The PCR product is 97%~98% homologous to ItuB gene of iturin A operon.This result shows that the antifungal mechanism of antagonistic Bacillus is related to Iturin group lipopeptin producing.There are no any PCR products appearing among 3 gram-negative bacteria.More research on their antifungal mechanism need to be done in the future. |
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| Keywords: | Ustilago scitaminea antagonistic bacteria Iturin A |
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