| 马铃薯天冬氨酸蛋白酶抑制剂基因5′上游调控区的分离和序列分析 |
| |
| 引用本文： | 刘中大,扈廷茂,王永胜. 马铃薯天冬氨酸蛋白酶抑制剂基因5′上游调控区的分离和序列分析[J]. 内蒙古大学学报(自然科学版), 1996, 0(4) |
| |
| 作者姓名： | 刘中大 扈廷茂 王永胜 |
| |
| 作者单位： | 内蒙古大学生物学系 |
| |
| 基金项目： | 内蒙古和国家自然科学基金 |
| |
| 摘 要： | 利用单一特异引物聚合酶链反应技术，将马铃薯ＤＮＡ中天冬氨酸蛋白酶抑制剂家族一成员的５′上游区分离，并克隆进ｐＵＣ１８质粒ＳｍａＩ位点中．用３２Ｐ中标记的单一特异引物──ＰｒｉｍｅｒＡ为探针，经斑点杂交得一阳性克隆．ＤＮＡ测序获得含有马铃薯天冬氨酸蛋白酶抑制剂基因５′上游区的插入物的核苷酸序列．从这序列结构中见到ＴＡ富含区，并发现典型的调控序列ＴＡＴＡ和ＣＡＡＴ．此上游序列与国外所得到的天冬氨酸蛋白酶抑制剂的上游区相比较在序列和ＴＡＴＡ及ＣＡＡＴ盒的位置上有着很大的不同．
|
| 关 键 词： | 马铃薯，天冬氨酸蛋白酶抑制剂基因，5′上游区单一特异引物聚合酶链反应 |
Isolation and Structural Analysis of the 5'-Upstream Promoter Region of an Aspartic Proteinase Inhibitor Gene from Potato |
| |
| Liu Zhongda, Hu Tingmao, Wang Yongsheng. Isolation and Structural Analysis of the 5'-Upstream Promoter Region of an Aspartic Proteinase Inhibitor Gene from Potato[J]. Acta Scientiarum Naturalium Universitatis Neimongol, 1996, 0(4) |
| |
| Authors: | Liu Zhongda Hu Tingmao Wang Yongsheng |
| |
| Abstract: | A DNA fragment containing the 5'-upsteam region of an aspartic proteinase inhibitor gene was isolated from potato total DNA by the technique of single-specific-primer polymerase chain reaction (ssp-PCR). Then the DNA fragment was cloned into the SmaI site of pUC18 vector.Screenlhg With the 32P-single-specific-Primer,Primer A,one positive clone was obtained. Typical CAAT and TATA box-like sequences were found in the insert containing the 5'-upsteam region of an aspartic proteinase inhibitor gene. |
| |
| Keywords: | potato aspartic proteinase inhibitor 5'-upsteam promoter region single-specific-primer PCR |
| 本文献已被 CNKI 等数据库收录！ |
