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| 红树林内生镰刀菌732#代谢产物Enniatin发酵条件研究 | |
| 引用本文： | 游剑岚,毛巍,周世宁,王军,林永成,吴思扬.红树林内生镰刀菌732#代谢产物Enniatin发酵条件研究[J].中山大学学报(自然科学版),2006,45(4):75-78. |
| 作者姓名： | 游剑岚 毛巍 周世宁 王军 林永成 吴思扬 |
| 作者单位： | 1. 中山大学生命科学院,广东,广州,510275 2. 中山大学药学院,广东,广州,510080;中北大学化学工程系,山西,太原,030051 3. 中山大学药学院,广东,广州,510080 4. 中山大学化学化工学院,广东,广州,510275 |
| 基金项目： | 国家自然科学基金;广东省自然科学基金;广东省博士启动基金 |
| 摘 要： | 红树林内生真菌732#可产生具有抗癌活性的新化合物enn iatin G。出于对enn iatin G的兴趣,通过ITS法测序分析,对732#种属进行了鉴定,得出732#属于Fusarium属真菌。为了增加732#的代谢产物量,以菌丝质量浓度(干质量)和enn iatin质量浓度为检测指标,用正交法确定了适合732#生长的最佳碳源、氮源及温度,再用单因素变换法对732#培养条件进行了优化,得出,在含w=2.5%葡萄糖,w=0.8%蛋白胨,起始值为pH6,20%人工海水的GTY培养基中发酵10 d,菌丝质量浓度(干质量)和enn iatin质量浓度可达最大值。 |
| 关 键 词： | 发酵条件 海洋内生真菌 |
| 文章编号： | 0529-6579（2006）04-0075-04 |
| 收稿时间： | 2005-10-08 |
| 修稿时间： | 2005-10-08 |
Fermentation Conditions and Characterization of Endophytic Fungus #732 Producting Novel Enniatin G from South China Sea |
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| YOU Jia-lan,MAO Wei,ZHOU Shi-ning,WANG Jun,LIN Yong-cheng,WU Si-yang.Fermentation Conditions and Characterization of Endophytic Fungus #732 Producting Novel Enniatin G from South China Sea[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2006,45(4):75-78. | |
| Authors: | YOU Jia-lan MAO Wei ZHOU Shi-ning WANG Jun LIN Yong-cheng WU Si-yang |
| Institution: | 1. School of Life sciences, Sun Yat-sen University, Guangzhou 510275 ,China; 2. School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou 510080, China; 3. Department of Chemical Engineering, North University of China, Taiyuan 030051, China; 4. School of Chemistry and Chemical Engineering , Sun Yat-sen University, Guangzhou 510275 ,China |
| Abstract: | The endophytic fungus 732#,isolated from the mangrove in the South China Sea,with anticancer novel Enniatin G,was characterized by ITS sequence analysis and phylogenetic analysis,which showed that the 732# belongs to the genus of Fusarium sp.To increase the content of the metabolites of the 732#,orthogonal test was used to select the optimum carbon sources and nitrogen sources with dry mycelium weight as observed objects;The incubation conditions for the growth and enniatin production of the Fusarium 732# were studied through changing the concentrations of the carbon sources and nitrogen sources,salt concentrations, and the initial pH values with enniatins and dry mycelium weight as observed objects.Under the optimal fermentation conditions,in GTY medium containing 25.% glucose,0.8% tryptone,pH 6,20 % artificial seawater and 10 days incubation,the maximal dry weight and enniatin yield were obtained. |
| Keywords: | enniatin G |
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