Structure of a bacterial 30S ribosomal subunit at 5.5 A resolution. |
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Authors: | W M Clemons J L May B T Wimberly J P McCutcheon M S Capel V Ramakrishnan |
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Affiliation: | Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84103, USA. |
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Abstract: | The 30S ribosomal subunit binds messenger RNA and the anticodon stem-loop of transfer RNA during protein synthesis. A crystallographic analysis of the structure of the subunit from the bacterium Thermus thermophilus is presented. At a resolution of 5.5 A, the phosphate backbone of the ribosomal RNA is visible, as are the alpha-helices of the ribosomal proteins, enabling double-helical regions of RNA to be identified throughout the subunit, all seven of the small-subunit proteins of known crystal structure to be positioned in the electron density map, and the fold of the entire central domain of the small-subunit ribosomal RNA to be determined. |
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