| 曲霉型糖化酶等电点的理论计算 |
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| 引用本文： | 冯晓勇,李济洪.曲霉型糖化酶等电点的理论计算[J].山西大学学报(自然科学版),1991,14(2):179-182. |
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| 作者姓名： | 冯晓勇 李济洪 |
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| 作者单位： | 山西大学分子科学研究所
(冯晓勇，郝军山，袁静明)，山西大学数学系(李济洪) |
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| 摘 要： | 该文介绍了一种根据蛋白质的氨基酸组成,应用计算机数据处理技术从理论上计算蛋白质、酶及多肽的等电点的新方法。和传统的生物化学研究方法相比,该法不仅简单、方便,而且结果可信度较高,除对已知其pI的蛋白质或酶的实测值印证外,还可预测蛋白质或酶的等电点。这在蛋白质化学、酶学等研究领域具有一定应用价值。文中计算了8种曲霉型糖化酶的pI,计算结果和文献报道基本吻合。
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| 关 键 词： | 糖化酶 曲霉型 等电点 预测 酶 |
THEORETICAL CALCULATION OF THE ISOELECTRIC POINTS OF Aspergillus GLUCOAMYLASES |
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| Feng Xiaoyong Hao Junshan Yuan Jingming Li Jihong.THEORETICAL CALCULATION OF THE ISOELECTRIC POINTS OF Aspergillus GLUCOAMYLASES[J].Journal of Shanxi University (Natural Science Edition),1991,14(2):179-182. |
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| Authors: | Feng Xiaoyong Hao Junshan Yuan Jingming Li Jihong |
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| Institution: | Feng Xiaoyong Hao Junshan Yuan Jingming Li Jihong |
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| Abstract: | In this paper, a new method for theoretical calculation of the isoelectric points (pI) of proteins is described using data processing technique, provided that their amino acid composition were known. With this method, the pIs of eight Aspergillus glucoamylases, one protein and one peptide can be predicted. The resuults show that pIs of prediction coincide well with those of experiment. |
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| Keywords: | prediction of isoelectric point Aspergillus glucoamylase |
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