基于疏肝解郁功效的逍遥散寒苦肝和温辛肝性效关系研究

为探讨逍遥散中疏肝解郁中药的寒温差异与其功效的内在联系,通过数据库检索获取寒苦肝和温辛肝药性的主要有效成分及作用靶点,基于STRING平台构建蛋白质-蛋白质相互作用网络,采用Metascape对识别出的药性组合蛋白质-蛋白质相互作用网络进行京都基因与基因组百科全书通路注释和统计分析。结果表明:逍遥散中归肝经药性主要通过HTR2B等靶点参与神经递质、信号通路、内分泌发挥疏肝解郁的功效特点;逍遥散中寒苦药性主要通过CREBRF等靶点参与能量代谢、炎症反应发挥疏肝解郁的功效特点;逍遥散中温辛组合药性主要通过ADCY5等靶点参与炎症反应神经营养、氨基酸代谢发挥疏肝解郁的功效特点。该研究从分子网络水平阐释了逍遥散中归肝经药性的共性特征和寒苦药性、温辛药性的特异性特征,为逍遥散的药性与功效的内在联系研究提供了新思路和新方法。

Nature-effect relationship research of cold bitter and warm pungent medicinal properties of Xiaoyaosan for soothing the liver and relieving depression based on nature combination

The purpose of this study was to explore the internal relationship between temperature differences and efficacy of Traditional Chinese Medicine Xiaoyaosan,used for soothing liver and relieving depression. The main active ingredients and targets of the combination of cold bitter and warm pungent medicinal properties were obtained by database retrieval. The protein-protein interaction network was constructed based on STRING platform, and Kyoto encyclopedia of genes and genomes pathway and statistical analysis of the identified medicinal properties' combination protein-protein interaction network were carried out using metascape. The results show that the liver meridian properties of Xiaoyaosan were mainly involved in neurotransmitter, signal pathway, and endocrine pathway through HTR2B and other targets, and the cold bitter medicinal properties of Xiaoyaosan were mainly involved in energy metabolism and inflammatory reaction through CREBRF and other targets. The warm pungent medicinal properties of Xiaoyaosan was mainly involved in inflammatory reaction, neuronutrition, and amino acid metabolism through ADCY5 and other targets, thereby soothing the liver and relieving depression. The study explained the common characteristics of the liver meridian medicinal properties and specific characteristics of cold bitter medicinal properties and warm pungent medicinal properties from the molecular network level in Xiaoyaosan, and provided new ideas and new methods for the study of the internal relationship between the medicinal properties and efficacy of Xiaoyaosan.