L-蛋氨酸缩邻香草醛Zn(Ⅱ)配合物对S .Pombe作用的热动力学研究

目的合成L-蛋氨酸缩邻香草醛Zn(Ⅱ)配合物,并通过微量热法研究配合物对S.Pombe的作用。方法以L-蛋氨酸、邻香草醛和氯化锌为原料合成了L-蛋氨酸缩邻香草醛Zn(Ⅱ)配合物,利用红外光谱、紫外光谱和元素分析等分析方法进行表征。于32℃条件下,采用微量热法测得不同浓度配合物作用下S.Pombe的生长热曲线(P-t),研究其最大产热功率P_(max)、生长速率常数k、传代时间tG、抑制率I等热动力学参数与配合物浓度c的定量关系,以及配合物的半抑制浓度IC50。结果随配合物浓度c的增大,S.Pombe的最大产热功率P_(max)呈直线递减趋势,生长速率常数k减小,传代时间tG增大,配合物的抑制率I增大,其半抑制浓度IC50为0.091 1g·L-1。结论该配合物对S.pombe生长有良好的抑制作用。

Thermokinetic studies of the effect of Zn(Ⅱ) complex with O-vanillin-L-methionine on the growth metabolism of S . Pombe

Purposes—To synthetize the Zn(Ⅱ) complex with O-vanillin-L-methionine and then to investigate the effect of the complex on S .Pombe with microcalorimetry .Methods—The Zn (Ⅱ) complex w as obtained by condensation reaction of L-methionine ,O-vanillinand zinc chloride ,then IR spectra ,UV spectra and elemental analysis were employed to characterize the structure .At the tem-perature of 32°C ,the P - t curves of S .pombe were obtained under different concentrations of the complex with a micro calorimeter .The maximum heat output power ( Pmax) ,microbial growth rate constant ( k ) and generation time ( tG) ,inhibition ratio ( I ) and half inhibition concentrate IC50were calculated .Results—The maximum heat output power( Pmax) and microbial growth rate constant( k ) decreased ,while generation time( tG) and inhibition ratio( I ) increased with the increase of concen-tration of complex ,the IC50value was found to be 0 .091 1 g · L -1.Conclusion—The complex has good action on the inhibition of the grow th of S .pombe .